Lajat, Sandrine, Simone Harbon, and Zahra Tanfin. Carbachol-induced desensitization of PLC-b pathway in rat myometrium: downregulation of Gqa/G11a. Am. J. Physiol. 275 (Cell Physiol. 44): C636–C645, 1998.—In the estrogentreated rat myometrium, carbachol increased the generation of inositol phosphates by stimulating the muscarinic receptorGq/G11-phospholipase C-b3 (PLC-b3) cascade. Exposure to c...

PURPOSE To examine the expression of prostaglandin (PG) receptors EP2, EP4, and FP in a human lens epithelial cell line (HLE-B3) at molecular and pharmacologic levels. METHODS Reverse transcription-polymerase chain reactions (RT-PCR) were performed with total RNA preparations from HLE-B3 cells using sense and antisense primers for each of the three prostaglandin receptors. The PCR products we...

Background: The HPA-1 alloimmune system carried by the platelet integrin aIIbb3 is the primary cause of alloimmune thrombocytopenia in Caucasians and the HPA-1b allele might be a risk factor for thrombosis. HPA-1a and -1b alleles are defined by a leucine and a proline, respectively, at position 33 in the b3 subunit. Although the structure of aIIbb3 is available, little is known about structural...

A recessive mutation hsi2 of Arabidopsis (Arabidopsis thaliana) expressing luciferase (LUC) under control of a short promoter derived from a sweet potato (Ipomoea batatas) sporamin gene (Spo(min)LUC) caused enhanced LUC expression under both low- and high-sugar conditions, which was not due to increased level of abscisic acid. The hsi2 mutant contained a nonsense mutation in a gene encoding a p...

Electric field (EF) exposure can affect the elongation, migration, orientation, and division of cells. The present study tested the hypothesis that EF may also affect epithelial‑mesenchymal transition (EMT) in lens epithelial cells and that this effect may be an important inducer in the pathological process of posterior capsule opacification (PCO). Human lens epithelial (HLE)‑B3 cells were expo...

Bacteriophage B3 is a transposable phage of Pseudomonas aeruginosa. In this report, we present the complete DNA sequence and annotation of the B3 genome. DNA sequence analysis revealed that the B3 genome is 38,439 bp long with a G+C content of 63.3%. The genome contains 59 proposed open reading frames (ORFs) organized into at least three operons. Of these ORFs, the predicted proteins from 41 OR...

PURPOSE This study was undertaken to investigate whether thioltransferase (TTase) exhibits dehydroascorbate (DHA) reductase activity in human lens epithelial cells. METHODS TTase was investigated for DHA reductase activity in vitro by the method of glutathione reductase-coupled spectrophotometric assay. DHA reductase activities of human lens epithelial (HLE-B3) cell lysate and TTase-depleted ...

The chance of developing lung cancer is increased through being exposed to cigarette smoke illustrated by studies. It is vital to understand the development of particular histologic-type cancers regarding the deposition of carcinogenic particles, which are present in human airway. In this paper, the mass transfer and deposition of cigarette smoke, inside the human airway, are investigated apply...