Transformation was used to perform ratio test crosses with mutant strains of Azotobacter vinelandii unable to fix N2. Mutations that simultaneously eliminated both components of nitrogenase (nif-1 and nif-2) were tightly linked. The nif-45 mutation that resulted in the absence of an active molybdenum cofactor was closer to nif-1 and nif-2 than to any of the other nif mutations. Strains that lac...

Micrographs of freeze-cleaved Azotobacter vinelandii cysts reveal that the surface is composed of several overlapping layers. This observation is consistent with the previously proposed structure of the outer cyst coat.

The present investigation the effect of different microbial culture on growth, yield and quality Sponge Gourd (Luffa cylindrica L.) was undertaken at Vegetable Research Farm, Department Horticulture, Naini Agricultural Institute, Sam Higginbottom University Agriculture, Technology & Sciences, Prayagraj (UP) during 2022. experiment laid out in a Randomized Block Design with 10 tratment viz.,...

The flavoprotein from Azotobacter, first isolated by SHETHNA et al. 1, is chemically well characterized, but its enzymatic function is still obscure. ARNON and coworkers 2 found this flavoprotein to be active in substituting for ferredoxin in the reduction of acetylene by a cell-free nitrogenase preparation from Azotobacter. We have recently confirmed their observation3. In addition, we could d...

Two outstanding characteristics of azotobacter, their abilitv to fix nitrogen and unusually high rates of oxidation, have stimulated interest in obtaining cell free extracts suitable for biochemical analysis of these processes. Many difficulties have been encountered in obtaining such extracts. Attempts to obtain cell free extracts capable of nitrogen fixation have given extremely inconsistent ...

In order to investigate the effect of Plant Growth Promoting Rhizobacteria (PGPR) and rhizobium bacteria on grain yield and some agronomic traits of mungbean (Vigna radiate L.), an experiment was carried out based on randomized complete block design with three replications in Malekshahi, Ilam province, Iran during 2012-2013 cropping season. Experimental treatments consisted of control treatment...

HIGHLIGHTS Rhizobacteria (Azotobacter spp.) have improved the quality and quantity of safflower seed protein.Protein quality was confirmed by SDS-PAGE and new bands were found in response to different combinations of rhizobacteria and lower doses of fertilizers.The PGPR application has reduced the use of fertilizers upto 50%. Protein is an essential part of the human diet. The aim of this prese...

Azotobacter vinelandii stimulated the growth of Agrobacterium tumefaciens H2, H23, H24, H27, and ATCC 15955 on media containing insoluble iron sources. The Azotobacter vinelandii siderophores appeared to promote Agrobacterium tumefaciens growth by solubilizing mineral iron, and the ferrisiderophores so formed then acted as iron sources for Agrobacterium tumefaciens. Agrobactin, the Agrobacteriu...

introduction: use of unbalanced chemical fertilizers especially p, having low absorption efficiency and low solubility compounds with soil components, has resulted in the production and use of bio-fertilizers (17, 23 and 29). bio-fertilizer is a preservative material consisting of one or several specific beneficial micro-organisms or their metabolic products used to supply plant nutrients and d...