Four types of utilization of nitrate nitrogen were observed with fourteen strains of the azotobacter. These were: (a) Those which utilize NO;-N with no or only a short lag; three strains of Azotobacter winelandii, a stock culture of A. chroococcum C-77, and seven strains of A. chrmoccum freshly isolated from soil were in this group; (b) A single strain, a stock culture of A. chroococcum C-44, w...

Goldschmidt, Millicent C. (University of Texas, Austin), and Orville Wyss. Chelation effects on Azotobacter cells and cysts. J. Bacteriol. 91:120-124. 1966.-Ethylenediaminetetraacetate (EDTA) is very toxic to Azotobacter in the presence of nitrogen compounds that form complexes with it. This appears to be due to stronger chelation of certain metal ions by the complex. When such complexes of EDT...

Two novel antibacterial peptides of clostridial species were purified, N-terminally sequenced, and characterized. Moreover, their structural genes were identified. Closticin 574 is an 82-amino-acid bacteriocin produced by Clostridium tyrobutyricum ADRIAT 932. The supernatant of the producing strain showed a high level of activity against the indicator strain C. tyrobutyricum. The protein is syn...

In order to evaluate the effects of inoculants and chemical fertilizer on quantitative and qualitative yield, a bread wheat cultivar treated with Azotobacter, arbuscular mycorrhiza fungi (AMF) and nitrogen sources by using split plot on the basis of randomized complete block design with three replications in Fars Agricultural Research Station during 2007-2008. Main plots consisted of nitrogen f...

Readily available inexpensive substrate with high product yield is the key to restoring acetone-butanol-ethanol (ABE) fermentation to economic competitiveness. Lactose-replete cheese whey tends to favor the production of butanol over acetone. In the current study, we investigated the fermentability of milk dust powder with high lactose content, for ABE production by Clostridium acetobutylicum a...

A beta-1,4-endoglucanase gene (eglA) cloned from C. acetobutylicum P262 was selected for use in the development of a reporter system for C. beijerinckii NCIMB 8052. The reporter plasmid, pER1, was constructed by ligating the promoterless eglA gene into the B subtilis/Clostridium shuttle vector, pFNK1, which can replicate and is stably maintained in C. beijerinckii. The expression of the endoglu...

Quantitative N,N,N',N'-tetramethyl-p-phenylenediamine (TMPD) oxidase and superoxide dismutase (SOD) analyses were performed on representative organisms of the family Azotobacteraceae. Azotobacter vinelandii, Azotobacter chroococcum, Azotobacter paspali, and Derxia gummosa exhibited high quantitative TMPD oxidase activities, and their extracts possessed very active and electrophoretically homoge...

Aerobic and microaerobic diazotrophs possess numerous oxygen restriction strategies to protect nitrogenase from inactivation by oxygen without interfering with energy generation through oxidative phosphorylation. Protection by conformational change in nitrogenase was first detected and described in Azotobacter. This strategy once considerd unique for Azotobacter has been shown in this study to ...

One of the problems in the production of microbial preparations for agriculture is the maintenance of bacterial viability. One of the methods of improving the viability is an immobilization in the carrier material. Immobilization of plant growth-promoting bacteria Streptomyces griseoviridis and Azotobacter sp. in the peat and experimental ceramic granules was studied. Bacterial viability in ste...