The activity of guanylyl cyclase (GC) responsible for cGMP synthesis was found to be predominantly located in a fraction of plasma membranes in cells of Avena sativa seedlings. The GC activity was shown to depend on light conditions of plant cultivation and seedlings age. The reversible regulation of the GC activity in etiolated seedlings by red/far red light was found. This suggests that photo...

This study was undertaken to evaluate natural mulches for weed control in organic onion (Allium cepa) production where current practices rely on hand-weeding or plastic mulch. Three experiments were conducted over 2 years, with two experiments conducted on-farm in different years and one experiment conducted on-station. Treatments consisted of hand-weeding or mulches of wheat (Triticum aestivum...

Polygalacturonase activity has been detected in a number of plants including seedlings of Phaseolus vulgaris, Zea mays, Avena sativa, and Pisum sativum. Particular emphasis was placed on characterizing the enzyme from oat seedlings. This enzyme is solubilized by 0.2 m NaCl, and its activity is highest near the apical tips of oat coleoptiles. It has a pH optimum between 5 and 5.5 and is activate...

Heat-bleached oat (Avena sativa L. cv Porter) leaves lacking 70S chloroplast ribosomes have been used to demonstrate that four chloroplast-localized enzymes of pyrimidine nucleotide biosynthesis: aspartate carbamoyl-transferase, dihydroorotase, orotidine phosphoribosyl-transferase, and orotidine-5'-phosphate decarboxylase, are synthesized on cytoplasmic ribosomes. Two other chloroplast enzymes,...

Methods for the post-translational control of protein function with light hold much value as tools in cell biology. To this end, we report a fusion protein that consists of DnaE split-inteins, flanking the light sensitive LOV2 domain of Avena sativa. The resulting chimera combines the activities of these two unrelated proteins to enable controlled formation of a functional protein via upregulat...

In attempt to elucidate the early molecular events associated with the biological action of cyclic guanosine 3/,5/monophosphate (cGMP) its binding to the structural components of Avena sativa L. plant cell has been studied. cGMP was shown to be bound specifically to proteins located predominantly in the soluble cytosolic fraction. cGMP-agarose affinity purification procedure followed by SDS-PAG...

Gravitropism of oat (Avena sativa L.) and wheat (Triticum aestivum L.) coleoptiles was investigated in relation to the displacement angle or to the initially set stimulation angle (SA). We measured curvature rates at the early phase of curvature, before it was affected by the drop in SA resulting from the curvature response itself. The plot of the rates against the sines of initial SAs revealed...

Two compost formulations based on oat straw (Avena sativa) and brachiaria (Brachiaria sp.) were tested for the cultivation of three Agaricus bisporus strains (ABI-07/06, ABI-05/03, and PB-1). The experimental design was a 2 × 3 factorial scheme (composts × strains) with 6 treatments and 8 repetitions (boxes containing 12 kg of compost). The chemical characterization of the compost (humidity, or...

Metal contaminants in soil environments derived from industrial pollution have clearly established the need for research on bioavailability and potential health risks. Much research has been conducted on metal sorption in soils. However, there is still a need to better understand the availability of metal contaminants to plants and microbes. Such information will enhance both human health and d...

Nanoparticles bio production, considering their performance in medicine and biologicalscience, is increasing; also, raising awareness of green chemistry and bioprocesses hasencouraged the use of environmentally friendly methods for the production of non-toxicnanomaterials.. Also uses of plant extracts are found to be more advantageous over chemical,physical and microbial (bacterial, fungal, alg...