In our group, we perform successfully MALDI by using femtosecond laser techniques in order to examine the ionization process. One recent result is MALDI with ultrashort laser pulses in the near IR (central wavelength of =800 nm) [2]. In this wavelength regime, used matrix substances have no absorption bands. Experiments with different laser pulse durations suggest that multi photon ionization i...

The fundamental work and current knowledge on the mechanisms of matrix-assisted laser desorption ionization with pulsed infrared lasers is reviewed and discussed, with a special emphasis on the work performed in the laboratory of the authors in the recent past. The role of the different relevant irradiation parameters (laser wavelength, laser pulse duration, irradiated area and laser fluence) a...

Proteomic expression patterns derived from mass spectrometry have been put forward as potential biomarkers for the early diagnosis of cancer and other diseases. This approach has generated much excitement and has led to a large number of new experiments and vast amounts of new data. The data, derived at great expense, can have very little value if careful attention is not paid to the experiment...

A new atmospheric pressure ionization method termed pyroelectricity-assisted infrared laser desorption ionization (PAI-LDI) was developed in this study. The pyroelectric material served as both sample target plate and enhancing ionization substrate, and an IR laser with wavelength of 1064 nm was employed to realize direct desorption and ionization of the analytes. The mass spectra of various co...

Synthetic host-guest chemistry is a versatile tool for biomedical applications. Characterization and detection of host-guest complexes in biological systems, however, is challenging due to the complexity of the biological milieu. Here, we describe and apply a mass spectrometric method to monitor the association and dissociation of nanoparticle (NP)-based host-guest interactions that integrates ...