نتایج جستجو برای: antigenemia
تعداد نتایج: 822 فیلتر نتایج به سال:
In this series, antilymphoid induction therapy did not appear to be necessary to prevent early graft loss from rejection. In addition, we have followed cytomegalovirus (CMV) antigenemia (pp65) for CMV infection. Although some patients developed a positive antigenemia in the seropositive to negative donor-recipient combinations, only one patient had a prolonged febrile course for 1 week.
Rationale: Patients receiving an allogeneic stem cell graft from cytomegalovirus (CMV) seronegative donors are particularly prone to CMV reactivation with a high risk of disease and mortality. Therefore we developed and manufactured a novel vaccine and initiated a clinical phase I trial with a CMV phosphoprotein 65 (CMVpp65)-derived peptide. Methods: Ten patients after allogeneic stem cell tran...
We demonstrated that sodium gluconate was the factor causing false-positive galactomannan (GM) antigenemia of Plasma-Lyte hydration solution. Infusion of sodium gluconate-containing solution but not gluconate-free Plasma-Lyte resulted in positive serum GM antigenemia. Serum GM concentrations also correlated with the volume and in vitro concentrations of GM within gluconate-containing solutions ...
Antigenemia in patients with Mediterranean visceral leishmaniasis (MVL) due to Leishmania infantum was retrospectively assessed by sandwich enzyme-linked immunosorbent assay (ELISA). Circulating Leishmania antigens, partially in free form, were in evidence in 53% of serum samples from immunocompetent individuals with MVL. Following successful therapy, antigenemia decline as measured by ELISA wa...
Abstract While RT-PCR tests of nasopharyngeal swabs remain the gold standard for detection SARS-CoV-2 infection and monitoring COVID-19 disease progression, measurement nucleocapsid antigenemia in serum plasma samples is an underexplored alternative proxy severity. To explore dynamics antigenemia, we measured levels antigen using a highly sensitive Single Molecule Array (Simoa) assay 817 serial...
background : toxoplasmosis is a worldwide endemic disease. in congenitally infected infants and aids patients, toxoplasmosis causes high rates of morbidity and mortality. in these cases antibody detection is difficult; so detection of parasite or its components could be useful tool for early detection and following treatment of the infection. methods : sixty-three balb/c mice were injected intr...
Fifty allogeneic stem cell transplant recipients were enrolled in a prospective cytomegalovirus pp65 antigenemia-guided preemptive therapy trial. Among these, 10 of 34 patients who received ganciclovir exhibited sustained and/or recurrent antigenemia despite treatment. Thirteen leukocyte preparations from these 10 subjects were screened for the presence of the most frequent cytomegalovirus UL97...
Cytomegalovirus (CMV) antigenemia was directly detected in polymorphonuclear leukocytes (PMNLs) from transplant recipients by using flow cytometry (FC). Two fixation and permeabilization methods and seven anti-CMV monoclonal antibodies (MAbs) were evaluated. 1C3, SL20, and NEA-9221 MAbs were more efficacious. The antigenemia detection threshold of FC was 0.05% positive PMNLs, and percentages co...
In the search for better protocols of preemptive therapy of human cytomegalovirus (HCMV) infection in hematopoietic stem cell transplant (HSCT) recipients, we conducted a randomized trial comparing antigenemia with the nucleic acid sequence-based assay (NASBA) for determination of HCMV immediate-early messenger RNA (IEmRNA) as the guiding assay for initiation of pre-emptive antiviral treatment....
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