The use of cationic biopolymer surfaces for high protein binding affinity matrices is described. As model proteins, fluorescently labeled bovine serum albumins (FITC-BSA, TRITC-BSA) have been employed. The amount of proteins on such cationically rendered surfaces was quantified by QCM-D. In addition, flexible, transparent, patterned COP slides have been prepared and loaded with proteins ranging...

three new rimantadine schiff bases (rsbs) were prepared, and then the interaction of rsbs with bovine serum albumin (bsa) was investigated using fluorescence, synchronous fluorescence, uv-vis absorption spectroscopy under physiological conditions. the results showed that the three rsbs effectively quenched the intrinsic fluorescence of bsa via static quenching. binding constant (ka), number of ...

Through observations of colloidal gold with silver enhancement, we have demonstrated that 2-nm colloidal gold labeled-testosterone-bovine serum albumin (BSA) conjugate or hydrocortisone-BSA conjugate injected intravenously enters the hormone-target cell nuclei of rats (Nishimura and Ichihara, 1997; Nishimura and Nakano, 1997, 1999). To confirm immunocytochemically whether the nature of BSA in t...

background: active immunization with gonadotropin releasing hormone conjugate (gnrh-bsa) manipulates the fertility axis and thus alters the reproductive cyclicity serum estradiol and progesterone levels. while the application of kamdhenu ark increases the efficacy of gnrh-bsa. objective: this experimental investigation is aimed to evaluate the modulatory effects on estrous cycle serum estradiol...

We reply to the criticisms of our procedure for determination of urinary albumin by centrifugal analysis (1). Gosling et at. (2) and Pejacovic and Peake (3) state that 5 mgfL is an appropriate figure for the detection limit. Using our method at both 340 and 440 nm, we assayed 21 replicate samples of a phosphate-buffered saline (PBS) blank and 1.25 mg/L human albumin standard in PBS. We demonstr...

kept at 29ºC for 10 days to allow the expression of the UAS constructs. Apc2 N175K is a loss-of-function allele, Apc Q8 is a null allele, UAS-Ras V12 and UAS-Tkv Q253 are gain-of-function transgenes. UAS-Tkv DN is a dominant negative transgene. Stocks were obtained form Bloomington Stock Center and VDRC. Staining and antibodies. Adult female flies were dissected in PBS. All the digestive tract ...

Three new rimantadine Schiff bases (RSBs) were prepared, and then the interaction of RSBs with bovine serum albumin (BSA) was investigated using fluorescence, synchronous fluorescence, UV-vis absorption spectroscopy under physiological conditions. The results showed that the three RSBs effectively quenched the intrinsic fluorescence of BSA via static quenching. Binding constant (Ka), number of ...

Three new rimantadine Schiff bases (RSBs) were prepared, and then the interaction of RSBs with bovine serum albumin (BSA) was investigated using fluorescence, synchronous fluorescence, UV-vis absorption spectroscopy under physiological conditions. The results showed that the three RSBs effectively quenched the intrinsic fluorescence of BSA via static quenching. Binding constant (Ka), number of ...

Studies were undertaken to determine whether BSA, once immobilized on activated nylon microspheres, would be capable of specifically removing circulating BSA antibody in vitro and in vivo in an extracorporeal circulation system in dogs. Nylon microspheres were prepared and, after gentle hydrolysis and glutaraldehyde treatment, demonstrated a retention of up to 34.5 mg of BSA. In vitro studies s...

Objective(s): The aim of this study was to synthesize triangular gold nanoparticles, and then to evaluate their capability for inhibition of Candida albicans secreted aspartyl proteinase 2(Sap2).Materials and Methods: To synthesize the nanoparticles, hydrogen tetrachloroaurate and hexadecyl trimethyl ammonium bromide were incubated in presence of Sn(IV) meso-tetra(N-methyl-4-pyridyl) porphine t...