The mean amylase level in 42 human bile samples was 154 IU/l and there was no significant difference in the amylase activity of 32 paired serum and bile samples. Estimation of the amylase thermolability of bile showed it to be similar to that of serum. This suggests that the amylase activity in bile may have filtered through the liver from the hepatic circulation rather than refluxed from the p...

The relative levels of pancreatic amylase mRNA closely parallel the 3-fold variation of pancreatic amylase protein levels observed in several mouse strains studied. In contrast pancreatic elastase mRNA levels were similar in these strains. Each of the strains contained the same number of amylase-like genes (about 8). The selective variation in the pancreatic amylase gene expression could be due...

The gene for beta-amylase was isolated from BacUlus polymyxa by molecular cloning in B. subtils. B. subtlis cells containing this gene express and secrete an amylase which resembles the B. polymyxa beta-amylase and barley beta-amylase in terms of the products it generates during carbohydrate hydrolysis. Starch hydrolysis with this beta-amylase produces maltose, not glucose, whereas maltotriose ...

In 1988, Hata et al. [1] reported a case of ectopic production of salivary-type amylase by an IgA-type multiple myeloma. Subsequently, seven cases of amylaseproducing multiple myeloma have been reported [2-8]. According to Delannoy et al. [7], amylase-producing myeloma should be added to the list of amylaseproducing tumors. In 1991, we reported a neuraminidase sensitive, so-called sialyl, saliv...

We describe a family of 32 subjects, including two healthy siblings without detectable pancreatic amylase in serum and five healthy relatives (one of them a sister) exhibiting subnormal pancreatic amylase activities. Comparisons of immunoreactive amylase concentrations with the corresponding amylase activities may justify the assumption of a "silent" Amy-2 allelic variant.

Li, Chenwei, Xuequn Chen, and John A. Williams. Regulation of CCK-induced amylase release by PKCin rat pancreatic acinar cells. Am J Physiol Gastrointest Liver Physiol 287: G764–G771, 2004. First published June 24, 2004; 10.1152/ajpgi.00111.2004.— PKC is known to be activated by pancreatic secretagogues such as CCK and carbachol and to participate along with calcium in amylase release. Four PKC...

BACKGROUND Alkaline α-amylases have potential applications for hydrolyzing starch under high pH conditions in the starch and textile industries and as ingredients in detergents for automatic dishwashers and laundries. While the alkaline α-amylase gains increased industrial interest, the yield of alkaline α-amylases from wild-type microbes is low, and the combination of genetic engineering and p...

Fungal alpha-amylase is an important occupational allergen in the bakery industry. Epidemiologic studies focusing on the relationship between alpha-amylase allergen exposure and work-related respiratory allergy, however, have not been reported yet. In this cross-sectional study, sensitization to occupational allergens and work-related symptoms were studied in 178 bakery workers and related to a...

Alpha amylase production using microbial source and solid state fermentation has been conducted for past few years in search of thermostable enzyme. Owing to the prolific use of thermostable alpha amylase in various industries like paper, food, detergent, brewing and starch liquefaction process, the production of alpha amylase is still going on. In the present work, Bacillus subtilis (ATCC 6633...

Stimulation of the beta-adrenergic or cholinergic muscarinic receptors are the principal mechanisms by which parotid salivary secretion is regulated in vivo. In this study we have examined the effects of cholinergic stimulation on amylase gene expression in dispersed rat parotid cells. [3H]Leucine incorporation into amylase and total protein was inhibited by carbamylcholine. Within 5 min of its...