AFLP is a novel high resolution fingerprinting method that can be used to delineate intraspecific relationships among a large variety of organisms, including bacteria. In the present study, this method was tested for its usefulness in the epidemiological typing of Acinetobacter strains. A total of 25 Acinetobacter strains originating from five hospital outbreaks in three countries were used. Is...

Phoenix dactylifera L. is a flowering plant species commonly known as the date palm and widely cultivated in most Middle East countries, including Libya. The present study analysed biochemical genetic diversity of fully mature eight Libyan cultivars grown different regions using amplified fragments length polymorphism (AFLP) technique. Six pairs AFLP molecular marker combinations were utilised ...

Whole-genome fingerprinting fluorescent amplified fragment length polymorphism (FAFLP) data were compared with in silico data for the sequenced strains of Mycobacterium tuberculosis (H37Rv and CDC1551). For this G+C-rich genome, many predicted fragments were not detected experimentally. For H37Rv, only 108 (66%) of the 163 predicted EcoRI-MseI fragments between 100 and 500 bp were visualized in...

Protocol: To detect the 506 polymorphism, a 567 bp SMPD1 genomic fragment is amplified using sense (5'-AGTAGTCGACATGGGCAGGATGTGTGG-3') and antisense (5'-AGTAGTGTCGACTTGCCTGGTTGAACC AC AGC-3') primers. Dot-blot hybridization is performed using allele-specific oligonucleotides for 506-Arg (5'-ACTACTCCAGGAGCTCT-3') and for 506-Gly (5'-ACTACTCCGGGAGCTCT-3'), which are hybridized at 42°C and washed ...

ABBREVIATIONS: AFLP (Amplified Fragment Length Polymorphism); BAC (Bacterial Artificial Chromosome); CAPS (Cleaved Amplified Polymorphic Sequence); CPCSD (California Planting Cotton Seed Distributors); EST (Expressed Sequence Tag); FOV (Fusarium oxysporum f. sp. vasinfectum); FW (Fusarium Wilt); QTL (Quantitative Trait Locus); RAPD (Random Amplified Polymorphic DNA); RIL (Recombinant Inbred Lin...