from normal mouse mammary gland showed some growthstimulating activity. SF from the kidney of tumor-bearing or multiparous old mice showed high activity, while that from tumor-free virgin young mice did not show significant activity. Hyperplastic alveolar nodules of the mammary gland showed activity as high as that of tumors. The growth-stimulating factor in the SF was precipitated with 60% sat...

The rapid growth in the use of electronic equipments, combined with early obsolescence has contributed enormously to the generation of large quantity of electronic (e) waste. One such e-waste, the mobile phone printed circuit boards (PCBs) contain various precious metals which can be extracted by different hydrometallurgical routes. The present work deals with the recovery of gold using ammoniu...

from normal mouse mammary gland showed some growthstimulating activity. SF from the kidney of tumor-bearing or multiparous old mice showed high activity, while that from tumor-free virgin young mice did not show significant activity. Hyperplastic alveolar nodules of the mammary gland showed activity as high as that of tumors. The growth-stimulating factor in the SF was precipitated with 60% sat...

We have developed an efficient chemoenzymatic synthesis of heparan sulfate oligosaccharides employing the para-nitrophenyl (p-NP) β-glucuronide as an acceptor compatible with enzymatic elongation and one that significantly simplifies oligosaccharide purification on C-18 resin. Employing ceric ammonium nitrate as oxidative reagent to remove the p-NP group unexpectedly also removed the glucuronic...

We have developed a method for the rapid extraction of nuclear proteins from cultured cells. The ammonium sulfate method described here extracts larger quantities of proteins that retain DNA binding activity than the modified Dignam method and another popular method used for the extraction of transcription factors. The ammonium sulfate method is rapid and can be used to process a large number o...

rpm). An inoculum of 5-8 % of the rusulting seed broth was then used to inoculate the fermentation flasks. The composition of the fermentation mediumwas as follows : sucrose 7 %, soybean meal 2.5 %, ammonium sulfate 0.3 %, pH 7.0, 25 ml of medium being used per 200ml flask. The fermentation was carried out on a rotary shaker at 200 rpm for about 10 days at 30°C. Feeding of S35-ammonium sulfate ...

Fog composition and deposition fluxes of fog water and fog solutes were measured in six radiation fog events in San Joaquin Valley, California during winter 1998/1999. Measurements made at 2 hr intervals with 0.30 m2 and 0.06 m2 Teflon deposition plates yielded excellent reproducibility (relative standard deviations of 3.8–6.0%) for water, nitrate, sulfate and ammonium. Water fluxes measured at...

In this study, the glutamate decarboxylase (GAD) gene from Lactobacillus brevis IFO12005 (Biosci. Biotechnol. Biochem., 61, 1168-1171 (1997)), was cloned and expressed. The deduced amino acid sequence showed 99.6% and 53.1% identity with GAD of L. brevis ATCC367 and L. lactis respectively. The His-tagged recombinant GAD showed an optimum pH of 4.5-5.0, and 54 kDa on SDS-PAGE. The GAD activity a...

In Florida, the dietary cation-anion balance (DCAD) characteristic of bahiagrass (Paspalum notatum) can be highly variable. This is a result of fluctuations in all four elements making up the core DCAD equation, Na, K, Cl, and S. Bahiagrass receiving annual contributions of S from ammonium sulfate fertilizer can accumulate high levels of S. In a previous study (Arthington et al. 2002), bahiagra...

Different samples of purified tobacco mosaic virus show a relatively wide variation in solubility in ammonium sulfate solution. This variation and the type of solubility curve obtained in the presence of varying amounts of solid phase show that the purified virus whether isolated by mild treatment with ammonium sulfate or by ultracentrifugation is not a homogeneous chemical substance but contai...