The relationship between alpha/beta and gamma/delta T-cell lineages was studied in rats using RT-PCR analysis of TCRbeta transcripts in gamma/delta T-cell hybridomas and an intracellular staining technique to detect TCRbeta protein in primary gamma/delta T-cells. We report the presence of functional TCRbeta transcripts in 2/9 gamma/delta T-cell hybridomas. About 15% of peripheral gamma/delta T-...

Infection of synchronized bovine fetal spleen cells with bovine parvovirus results in changes in the levels and patterns of DNA polymerases alpha and gamma during the cell cycle. The pattern of DNA polymerase alpha activity closely paralled viral DNA synthesis and the production of progeny virus, and levels, of this enzyme were threefold greater than in mock-infected cells during the period of ...

INTRODUCTION The Japan Nuclear Cycle Development Institute (JNC) carried out research and development projects related to uranium exploration in Japan from 1967 to 1987. Several mining facilities have been retained after the end of projects. Hence, the study on the behavior and the environmental impact of radon and its progeny from the mining facilities is being continued at the Ningyo-toge Env...

CD34+ precursors in normal human bone marrow (BM) generate large numbers of dendritic cells alongside macrophages and granulocytic precursors when cultured for 12 to 14 days in c-kit ligand, granulocyte-macrophage colony-stimulating factor (GM-CSF), and tumor necrosis factor-alpha (TNF-alpha). This study reports an intermediate cell type that develops by day 6, and has the potential to differen...

As information on indoor air quality accumulated* it became apparent that radon and its progeny are invariably present in indoor environments and that concentrations may reach unacceptably high levels. The lung cancer excess anong miners exposed to radon progeny raised concern that exposure to radon progeny might also cause lung cancer in the general population. This presentation first provides...

The sensitivities (in m) of bare LR115 detectors and detectors in diffusion chambers to 222Rn and 220Rn chains are calculated by the Monte Carlo method. The partial sensitivities of bare detectors to the 222Rn chain are larger than those to the 220Rn chain, which is due to the higher energies of alpha particles in the 220Rn chain and the upper energy limit for detection for the LR115 detector. ...

The use of immunoassays has facilitated the measurement of high molecular weight sensitizers, usually protein molecules, in the picogram and nanogram per cubic meter range. This facilitated the evaluation of exposure response relationships for bakery workers, exposed to wheat allergens and fungal alpha-amylase and other groups exposed to other allergens such as laboratory animal workers. The ap...

This study utilizes instruments from the Curiosity rover payload to develop an integrated paleoenvironmental and compositional reconstruction for 65-m thick interval of stratigraphy comprising Hartmann's Valley Karasburg members Murray formation, Gale crater, Mars. The consists cross-stratified sandstone (Facies 1), planar-laminated 2), mudstone 3). Facies 1 is composed showing truncated sets c...

PURPOSE To demonstrate the presence of corneal stromal precursors that express neural markers in vitro. METHODS To isolate sphere-forming cells, human corneal stromal cells were subjected to a reaggregation-free neurosphere assay in medium containing methylcellulose gel matrix. To promote differentiation, the isolated sphere colonies were plated in wells with medium containing fetal bovine se...