A hamster cell line resistant to alpha-amanitine has been isolated (alpha-am-r, BHK-T6-G-1). Cell extracts of this mutant have an alpha-amanitine-resistant RNA polymerase II (nucleosidetriphosphate: RNA nucleotidyl-transferase, EC 2.7.7.6) activity as shown by DEAE-cellulose column chromatography. This mutation is dominant in interspecific hybrids with 3T3 mouse cells. In such hybrids polyoma v...

The structure of cellulose microfibrils in wood is not known in detail, despite the abundance of cellulose in woody biomass and its importance for biology, energy, and engineering. The structure of the microfibrils of spruce wood cellulose was investigated using a range of spectroscopic methods coupled to small-angle neutron and wide-angle X-ray scattering. The scattering data were consistent w...

Purpose: Alpha-cellulose obtained as pulp from fibrous plant materials has found use in the pharmaceutical industry as a disintegrant and direct compression diluent. The aim of this study was to evaluate the suitability of α-cellulose obtained from waste paper as a direct compression excipient. Method: The flow and compaction characteristics of α-cellulose (ACP) obtained from waste paper, in co...

In the presence of actinomycin D or a combination of actinomycin D and either camptothecin or alpha-amanatin. Aedes albopictus cells synthesize a variety of single stranded RNA species. These actinomycin D resistant species are ethidium bromide sensitive and they are present in the cell cytoplasm in an RNase resistant structure which has the sedimentation and buoyant density characteristics of ...

Cotton dusts contain condensed tannins and endotoxins, which are suspected of contributing to the development of acute and chronic biological responses in some cotton textile mill workers. Condensed tannin extracted from cotton dust was coated on to cellulose powder, and the tannin coated powder was treated with an alkali solvent system previously developed to reduce the endotoxin content and p...

The white rot basidiomycete, Phanerochaete chrysosporium, employs an array of extracellular enzymes to completely degrade the major polymers of wood: cellulose, hemicellulose and lignin. Towards the identification of participating enzymes, 268 likely secreted proteins were predicted using SignalP and TargetP algorithms. To assess the reliability of secretome predictions and to evaluate the usef...

The alpha-particle sensitive colorless cellulose nitrate films (commercially available as LR 115 films from DOSIRAD, France) have been proposed as cell-culture substrates for alpha-particle radiobiological experiments. Cytocompatibility of the substrate is a key factor to the success of such experiments. The present work aims to investigate the cytocompatibility of surface-treated cellulose nit...

The interaction of the 1 alpha,25-dihydroxyvitamin D3 receptor with RNA and synthetic polynucleotides has been examined by using receptor from rachitic chicken intestine. Total intestinal RNA inhibited the binding of receptor to calf thymus DNA-cellulose with an efficiency equivalent to single-stranded DNA. A comparison of the inhibitory activity of several polyribonucleotides gave the followin...

Although intensive efforts have been made to create recombinant cellulolytic microorganisms, real recombinant cellulose-utilizing microorganisms that can produce sufficient secretory active cellulase, hydrolyze cellulose, and utilize released soluble sugars for supporting both cell growth and cellulase synthesis without any other organic nutrient (e.g., yeast extract, peptone, amino acids), are...

This research aimed to analyze the change in chemical composition of betung bamboo (Dendrocalamus asper) strands after alkali immersion treatment under various times. The culms were converted into with target length, width, and thickness 70, 25, 0.5 mm, respectively. Strands immersion-treated 1% NaOH solution for 1, 2, 3 h. Preparation powder component analysis refers Technical Association Pulp...