PREMISE OF THE STUDY Microsatellite loci were developed as polymorphic markers for the New Zealand endemic Myosotis pygmaea species group (Boraginaceae) for use in species delimitation and population and conservation genetic studies. METHODS AND RESULTS Illumina MiSeq sequencing was performed on genomic DNA from seedlings of M. drucei. From trimmed paired-end sequences >400 bp, 484 microsatel...

Expressed sequence tags (ESTs) can be used to identify microsatellite markers. We developed 30 polymorphic microsatellite markers from 5053 ESTs of the Miichthys miiuy. Out of 123 EST derived microsatellites for which PCR primers were designed, 30 loci were polymorphic in 30 individuals from a single natural population with 2-13 alleles per locus. The observed and expected heterozygosities were...

The genotypes for calpastatin (CAST) and calpain (CAPN) loci were determined by PCR-RFLP and PCRSSCPmethods. Blood samples were collected from 200 pure-bred Zel sheep from Shirang’s Zel sheep Breeding Station located in south-west of Golestan, Iran. Extraction of genomic DNA was performed based on the modified salting out method. Based on results, two investigated loci were polymorphic an...

PREMISE OF THE STUDY Microsatellite loci were developed for Afzelia quanzensis (Fabaceae) as a first step toward investigating genetic diversity and population structure of the species in its native range. METHODS AND RESULTS Illumina shotgun sequencing was used to generate raw sequence reads, which were searched for potential microsatellite loci. A total of 70 potential microsatellite loci w...

Genetic analysis of 30 accessions of marvel grass (Dichanthium annulatum Forsk.), a tropical range grass collected from grasslands and open fields of drier regions, was carried out with the objectives of identifying unique materials that could be used in developing the core germplasm for such regions as well as to explore gene (s) for drought tolerance. Five inter-simple sequence repeat (ISSR) ...

Genetic variation of the endangered Catalonian donkey breed (Equus asinus) has been analysed at 19 loci including seven protein loci and 12 microsatellite loci isolated from the domestic horse, in 98 individuals of both sexes. Only four protein markers and three microsatellites were polymorphic. Allele frequencies of the analysed loci showed close agreement with Hardy}Weinberg proportions, with...

japanese plum (prunus salicina lindl.) having originated from china, is now one of the important fruit trees in many countries in the world. knowledge of genetic diversity among existing germplasms provides the possibility of parental selection in breeding improvement programs. for assessment of genetic diversity and relationship among genotypes in iran (ramsar, velyan karaj and mashhad) and fo...

the kermani sheep is an important meat producing an animal in iran and its economic efficiency is mainly dependent on its growth and reproduction ability. inter-simple sequence repeat (issr) is the genome region between microsatellite loci. the aim of this study was to evaluate the association of two different issr markers with predicted breeding values (pbv) of body weight trait in kermani she...

We developed nine new nuclear dinucleotide microsatellite loci for Castor canadensis. All loci were polymorphic, except for one. The number of alleles ranged from two to four and from five to 12 in populations from Arizona and Wisconsin, respectively. Average heterozygosity ranged from 0.13 to 0.86 per locus. Since cross-species amplification in Castor fiber was successful only in four loci, we...

PREMISE OF THE STUDY Chloroplast microsatellite loci were characterized from transcriptomes of Artocarpus altilis (breadfruit) and A. camansi (breadnut). They were tested in A. odoratissimus (terap) and A. altilis and evaluated in silico for two congeners. METHODS AND RESULTS Fifteen simple sequence repeats (SSRs) were identified in chloroplast sequences from four Artocarpus transcriptome ass...