In this study, we investigated the possible use of novel lipidated sorbitol-based transporters as functional devices for the improvement of non-viral gene delivery. These transporters are composed of a sorbitol scaffold bearing 8 guanidine moieties that mimic the arginine residues of well-known cell-penetrating peptides. In addition, the transporters carry different lipid groups to aid DNA cond...

results responsiveness, effectiveness, and flexibility with eigenvalue of 2.628 were identified as the main aspects of agility in humanitarian health supply chains. the components of visibility, reactivity, and speed were identified as components of responsiveness. quality, reliability, and completeness were identified as components of effectiveness. volume, delivery, mix, and product were know...

background: the poor permeability of the plasma and nuclear membranes to dna plasmids are two major barriers for the development of these therapeutic molecules. therefore, success in gene therapy approaches depends on the development of efficient and safe non-viral delivery systems. objectives: the aim of this study was to investigate the in vitro delivery of plasmid dna encoding hpv16 e7 gene ...

Polypeptides were the first set of materials considered for use as nonviral gene delivery vectors. With its ability to bind and condense anionic plasmid DNA, cationic poly-l-lysine (PLL) was one of the most well studied of the early gene delivery polypeptides. Unfortunately, as a DNA delivery vector, unmodified PLL suffered from low transfection efficiency. Although there have been tremendous e...

Background: The poor permeability of the plasma and nuclear membranes to DNA plasmids are two major barriers for the development of these therapeutic molecules. Therefore, success in gene therapy approaches depends on the development of efficient and safe non-viral delivery systems. Objectives: The aim of this study was to investigate the in vitro delivery of plasmid DNA encoding HPV16 E7 gene...

The use of cationic polymers in gene therapy aims at improving the circulation, protection and delivery nucleic acids. modification poly(L-lysine) (PLL), including insertion hydrophobic moieties, is an efficient strategy to enhance transfection efficiency this synthetic system. PLL usually modified through either extremities polymer chains or amino groups present on side chains. Herein synthesi...

The key principle of gene delivery to articulations by direct intra-articular injection is to release complementary DNA(cDNA)-encoding medical products that will lead to maintained, endogenous production of the gene products withinthe articulation. In fact, this has been accomplished for both in vivo and ex vivo gene delivery, using several vectors,genes, and cells in some animal models. Some c...

Nowadays the application of nanotechnology in different biomedical fields such as drug delivery is increasing due to its unique advantages. With this in mind, it is widely believed that nanogels as the nanometer-sized networked polymeric particles have a considerable impact on drug delivery systems as biocompatible nanocarriers due to their unique characteristics such as high loading capacity, ...

There has been great interest recently in therapeutic use of nucleic acids including genes, ribozymes and antisense oligonucleotides. Despite recent improvements in delivering antisense oligonucleotides to cells in culture, nucleic acid-based therapy is still often limited by the poor penetration of the nucleic acid into the cytoplasm and nucleus of cells. In this report we describe nucleic aci...