Four structural polypeptides of Hazara virus, an agent closely related to the Crimean-Congo haemorrhagic fever (C-CHF) viruses, were resolved by SDS-polyacrylamide gel electrophoresis. Three glycoproteins were identified (mol. wt. 84,000, 45,000 and 30,000) and were found to be associated with the virion envelope. A fourth polypeptide (mol. wt. 52,000) was non-glycosylated and associated with t...

Dengue virus and dengue haemorrhagic fever are amongst the most important challenges in tropical diseases due to their expanding geographical distribution, increasing outbreak frequency, hyperendemicity and evolution of virulence. Here, the use of a RT-nested PCR for both the diagnosis and genetic characterization of dengue infections in clinical samples is described.

Infection with dengue viruses produces a spectrum of clinical illness ranging from a nonspecific viral syndrome to severe and fatal haemorrhagic disease. Important risk factors include the strain and serotype of the infecting virus, as well as the age, immune status, and genetic predisposition of the patient. The teaching point in this case study was Dengue fever which occurred concomitantly wi...

A disease causing increased mortality and abortion in domestic animals during a 1977 epizootic in Egypt was identified as Rift Valley fever (RVF) virus. The epizootic included extensive human involvement reflected clinically as either an acute febrile, ocular, encephalitic, or fatal haemorrhagic form of RVF disease. The virus was again isolated from humans and animals during a second RVF epizoo...

Dengue is an infectious mosquito-borne viral disease. Dengue or dengue-like epidemics ranks highly among new and newly emerging infectious diseases in public health significance and may affect persons of all ages in dengue endemic area. Dengue virus infections Montip Tiensuwan and Timothy O’Brien 2 may lead to dengue fever (DF), dengue haemorrhagic fever (DHF) and may lead to hypovolaemic shock...

Bats can carry important zoonotic pathogens. Here we use a combination of next-generation sequencing and classical virus isolation methods to identify novel nairoviruses from bats captured from a cave in Zambia. This nairovirus infection is highly prevalent among giant leaf-nosed bats, Hipposideros gigas (detected in samples from 16 individuals out of 38). Whole-genome analysis of three viral i...

Necropsies were carried out on two patients who died of Congo-Crimean haemorrhagic fever (C-CHF) in Dubai. The diagnosis was confirmed by isolation of C-CHF virus from the liver. Histopathological changes included extensive cellular necrosis and haemorrhage in the liver, necrosis and lymphoid depletion in the spleen, congestion and oedema formation in the lungs, and haemorrhage in a number of o...

BACKGROUND In the present study, a series of N-substituted acridone derivatives was synthesized and evaluated against two haemorrhagic fever viruses (HFV). METHODS Compounds were tested against Junin virus (JUNV), an arenavirus agent of Argentine haemorrhagic fever, and dengue virus (DENV), a flavivirus agent of the most prevalent arthropod-borne viral disease in humans. RESULTS Among teste...