UNLABELLED The canalicular plasma membrane is constantly exposed to bile acids acting as detergents. Bile acids are essential to mediate release of biliary lipids from the canalicular membrane. Membrane microdomains (previously called lipid rafts) are biochemically defined by their resistance to detergent solubilization at cold temperature. We aimed to investigate the canalicular plasma membran...

Solubilization of membrane proteins by amphiphilic detergents represents a crucial step in studies of membrane proteins in which proteins and lipids in natural membranes are dissociated giving rise to mixed clusters of proteins, lipids and detergents in the aqueous dispersion. Although solubilization is a popular method, physicochemical principles underlying solubilization are not well understo...

Rates of pH-dependent micellization of multilamellar vesicles by the hydrophobic polyelectrolyte poly(2-ethylacrylic acid) (PEAA) have been measured turbidometrically. This polymer shows a strong ph-dependence in its affinity for phospholipid membranes, binding in increasing amounts as pH is lowered and ultimately solubilizing membranes to form mixed micelles (Tirrell, Takigawa and Seki (1985) ...

The performance of an anaerobic digestion fed with waste activated sludge (WAS) pretreated with NaOH was examined. The sludge was periodically collected from a municipal wastewater-treatment plant. The laboratory work was run in four 1-1 semi-continuous anaerobic digestion reactors. Reactor A was fed with untreated WAS at 1% total solids (TS). The other three reactors, B, C and D, were respecti...

In this study, alkaline peroxide oxidation pretreatment was evaluated for sugarcane bagasse, a lignocellulosic biomass. By comparing the effects of NaOHH2O2 and Ca(OH)2 on pretreatments at specified reaction time periods (3, 6, 9, and 12 h) and reaction temperatures (60, 70, 80, and 90 h), optimum responses in term of cellulose content, hemicellulose solubilization, and lignin removal were esta...

pluronic/bile salt/phospholipid mixed micelles (pluronic/bs/ps-mm) drug carrier system for solubilization hydrophobic drugs was developed. a typical hydrophobic compound, pyrene, was selected as a representative hydrophobic compound to model the hydrophobic drugs. five pluronics, f68, f88, f98, f108, and f127 with different ppo chain length were studied. cmc data and solubilization capacities w...

Two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) is the common method of choice for proteomic analysis. By introducing several small changes, a method was developed that not only improved the resolution and reproducibility of 2D-PAGE but also shortened the time of analysis. Precipitation by alkaline phenol and methanol/ammonium acetate was the choice for protein extraction. However,...