When haemoglobin is added to human plasma, it is bound through the globin portion of the molecule to an C2-globulin (Jayle, Boussier & Badin, 1952; Nyman, 1959). It has also been suggested that in human sera haemoglobin combines with albumin (Neale, Aber & Northam, 1958) and with a Plglobulin (Neale et al. 1958; Tombs, 1960), but these complexes could not be found by Allison & ap Rees (1957), A...

The macrocyclic ligands 2,3,5,6-Bis{3’-bromo-5’-tert-butyl benzo-5-phenyl benzo} 12C3 (L1), 2,3,5,6-bis{3’-bromo-5’-tert butyl-benzo-5’-phenyl-benzo}15C4 (L2), 2,3,5,6-bis{3’-bromo-5’-tert butyl-benzo-5’-phenyl-benzo}18C5 (L3) have been synthesized and their alkaline metal compounds with NaClO4∙H2O and KClO4  have ...

The mode of membrane attachment of the Rieske iron-sulfur proteins from cytochrome b6f complex of pea thylakoids and from cytochrome bc1 complex of yeast mitochondria has been studied using biochemical approaches. The relative sensitivity of the Rieske protein to trypsin in the thylakoid membrane shows that all trypsin sites of the Rieske protein are on the lumen side of the thylakoid membrane....

Dissociation kinetic analysis of a complex of Ce(3+) with a polyaminocarboxylic ligand, 8-amino-2-[(2-amino-5-methylphenoxyl)methyl]-6-methoxyquinoline-N,N,N',N',-tetraacetic acid (Quin2), was studied by microchip capillary electrophoretic reactor. Dissociation rate constants, k(d), of Ce(3+)-Quin2 complex in alkaline conditions at pH 8.3 - 9.8 were determined. The linear relationship of k(d) w...

Using immunoelectrophoresis and other techniques, we have demonstrated an association between lipoprotein-X and (a) alkaline phosphatase and (b) other enzymes originating from the hepatocyte membrane, namely gamma-glutamyltransferase and leucine aminopeptidase. The high-molecular-mass forms of these enzymes, in both serum and bile, were precipitated by lipoprotein-X antiserum but not by antiser...