In this paper, we fabricated soft 3D bio devices utilizing PELID (Patterning with Electrostatically-Injected Droplet) method. It is preferable to perform laboratory experiments with 3D structures in bioengineering. We have investigated mechanism and fundamental characteristics of the PELID method and now been applying for new printing technology of high image quality and 3D printing technology....

Alginate hydrogel is a popular biologically inert material that is widely used in 3D bioprinting, especially in extrusion-based printing. However, the printed cells in this hydrogel could not degrade the surrounding alginate gel matrix, causing them to remain in a poorly proliferating and non-differentiating state. Here, we report a novel study of the 3D printing of human corneal epithelial cel...

This study reports an industrially applicable non-sterile xylitol fermentation process to produce xylitol from a low-cost feedstock like corn cob hydrolysate as pentose source without any detoxification. Different immobilization matrices/mediums (alginate, polyvinyl alcohol, agarose gel, polyacrylamide, gelatin, and κ-carrageenan) were studied to immobilize Candida tropicalis NCIM 3123 cells fo...

Freeze-drying is often used as a final stage to produce three-dimensional porous matrices for medicine. Because pure solvent crystallizes first during freezing, it acts pore-forming agent. The size of the crystals primarily depends on cooling rate and composition material be frozen. Ultrasonic treatment also affects can control structure matrix. This article describes effect ultrasound (40 kHz,...

Tooth loss is a common result of a variety of oral diseases due to physiological causes, trauma, genetic disorders, and aging and can lead to physical and mental suffering that markedly lowers the individual's quality of life. Tooth is a complex organ that is composed of mineralized tissues and soft connective tissues. Dentin is the most voluminous tissue of the tooth and its formation (dentino...