Hypertension is one of the most common worldwide diseases in humans. Angiotensin I-converting enzyme (ACE) plays an important role in regulating blood pressure and hypertension. An evaluation was done on the effect of Alcalase hydrolysis of defatted Jatropha curcas kernel meal on ACE inhibitory activity in the resulting hydrolysate and its purified fractions. Alcalase exhibited broad specificit...

Potential antioxidative activities of enzymatic extracts from seven species of brown seaweeds were evaluated using four different reactive oxygen species (ROS) scavenging assays containing DPPH (1,1-diphenyl-2-pricrylhydrazyl) free radical, superoxide anion, hydroxyl radical and hydrogen peroxide scavenging assay. The brown seaweeds were enzymatically hydrolyzed to prepare water-soluble extract...

Synthetic angiotensin I-converting enzyme (ACE-I) inhibitors can have undesirable side effects, while natural inhibitors have no side effects and are potential nutraceuticals. A protein-rich fraction from chia (Salvia hispanica L.) seed was hydrolyzed with an Alcalase-Flavourzyme sequential system and the hydrolysate ultrafiltered through four molecular weight cut-off membranes (1 kDa, 3 kDa, 5...

The aim of this work was the purification and identification of the major angiotensin converting enzyme (ACE) inhibitory peptides produced by enzymatic hydrolysis of a protein concentrate recovered from a cuttlefish industrial manufacturing effluent. This process consisted on the ultrafiltration of cuttlefish softening wastewater, with a 10 kDa cut-off membrane, followed by the hydrolysis with ...

Malaysia is one of the main producers duck meat globally with increasing demands. Increasing in production will also increase number by-products such as skin, feet and bones. Pekin (Anas platyrhyncos domestica) famous breeds. Gelatin that was extracted from has shown a potential raw material for bioactive peptide can involve various functions organism physiologically example antioxidant effects...

The study aimed to investigate the effects of alcalase, papain, flavourzyme, and neutrase on structural characteristics bioactivity stability Cucumaria frondosa intestines ovum hydrolysates (CFHs). findings revealed that flavourzyme exhibited highest hydrolysis rate (51.88% ± 1.87%). At pH 2.0, solubility hydrolysate was lowest across all treatments, while at other levels over 60%. primary stru...

Hemp (Cannabis sativa L.) seeds have recently been attracting attention as a new high-value-added food material owing to their excellent nutritional properties, and research on the development of functional materials using hemp is actively progressing. This study aimed evaluate antioxidant properties seed protein hydrolysates. Protein hydrolysates were prepared from defatted powder (HS) by enzy...

This is the first report about flaxseed protein hydrolysates applied as media supplements in CHO cell culture. The were produced by three separate enzymatic digestions of proteins isolated from oil cake. enzymes used Alcalase, Neutrase, and Protamex, most efficient hydrolysis was achieved with Alcalase. tested a partial substitute for serum basal order to evaluate their effects on adherent IgG-...

Here, we describe peptide aggregation, which is also known as enzymatic protein resynthesis. Whey protein hydrolysate (WPH) is the starting material for assembling peptides. Analyses of the involved amino acids, intrinsic fluorescence, fluorescence phase diagram, secondary structure, turbidity, and surface hydrophobicity were performed to investigate the reaction process. The aggregation mechan...