Brassica napus is a species of high agronomic interest, used as model to study different processes, including microspore embryogenesis. The DH4079 and DH12075 lines show low embryogenic response, respectively, which makes them ideal the basic mechanisms controlling embryogenesis induction. Therefore, availability protocols for genetic transformation these two backgrounds would help generate too...

Crown gall of walnut (Juglans sp.), caused by the bacterium Agrobacterium tumefaciens, greatly impacts the CA walnut industry. To determine the genetic diversity of A. tumefaciens throughout the Central Valley of CA, we collected isolates from 12 walnut growing counties. A total of 340 A. tumefaciens biovar 1 isolates were collected and tested for the presence of the Ti plasmid by PCR; 267 isol...

The Agrobacterium-mediated transformation of hairy roots creates a rapid and simple means to introduce and express foreign genes in plant cells that are capable of synthesizing specific natural products. We describe a method for the production of transgenic hairy root cultures of peanut (Arachis hypogaea L.) using Agrobacterium rhizogenes R1000, a strain with the binary vector pBI121. We evalua...

Several strains of Agrobacterium tumefaciens and A. rhizogenes were shown to form tumors on runners of the diploid strawberry species Fragaria vesca L. Tumors, weighing from 0.1 to 8.3 mg, appeared from 2 to 4.5 weeks after infection. The majority of tumors tested for opine synthesis by high-voltage paper electrophoresis analysis showed positive results. These results demonstrate that diploid s...