Three commercially available rapid slide agglutination tests for the identification of Staphylococcus aureus were evaluated with 354 recent clinical isolates (165 strains of S. aureus). The test results of two latex agglutination products, SeroSTAT Staph (Scott Laboratories, Inc.) and Staphylatex (American Micro Scan), and one hemagglutination product, Staphyloslide (BBL Microbiology Systems), ...

Febrile agglutination tests were done by using as antigens Brucella abortus, Salmonella group D, Proteus OX19, and Pasteurella tularensis. Comparison of results from 23 sera showed that the microtechnique, rapid slide, and test tube methods gave similar titers, although those from the microtechnique were generally higher. The sensitivity of the microtechnique depended upon the concentration of ...

Many of the commercial slide agglutination tests for Staphylococcus aureus incorporate antibodies against cell surface antigens associated with methicillin resistance, including capsular polysaccharides and an uncharacterized antigen, serotype 18. These tests are more sensitive than the first-generation agglutination procedures that detected only bound coagulase and protein A, but they suffer f...

Kodama et al. (9) recently described a passive agglutination test using Toraysphere particles and made a comparative evaluation with conventional Ratz-Randall and NA-Latex-ASC (Hoechst) methods to measure anti-streptolysin O (ASO) titer for the diagnosis of group A streptococcal (GAS) infections such as pharyngitis and scarlet fever, and of rheumatic fever and glomerulonephritis as a consequenc...

Vaginal swabs from women who on clinical evidence were thought to have vaginal candidosis were examined for yeasts by conventional laboratory methods (microscopy and culture) and also assayed for Candida antigens using a rapid (3 min) slide latex particle agglutination tests. Results showed that a diagnosis of vaginal candidosis based on clinical criteria alone is unreliable: only half of the w...

BACKGROUND Leptospirosis is often treated based on clinical diagnosis. There is a need for rapid laboratory diagnosis for this condition. The aim of this study was to compare the diagnostic accuracy of two rapid IgM based immunodiagnostic assays with the microscopic agglutination test (MAT), in acute leptospirosis infection. METHODS MAT, IgM based immunochromatographic test (Leptocheck-WB) an...

The authors evaluated a simple latex agglutination test for the serodiagnosis of acute human leptospirosis. A total of 380 serum samples from 85 confirmed leptospirosis patients and 202 non-leptospirosis patients were selected Using the selected cut-off value of weakly positive, the overall sensitivity and overall specificity of the test were 94.1% and 97.0% respectively. The weighted kappa val...

Rotavirus strains circulating in Sousse, Tunisia, between 1995 and 1999 were characterized antigenically by monoclonal antibodies to the VP6 subgroup and the VP7 serotype. The VP4 genotype was determined by reverse transcription-PCR, as were the strains with untyped VP7. Only 17% of 375 children were shedding rotavirus as determined by latex agglutination assay. Most rotavirus strains were G1P[...

Use of the Vi agglutination test in the detection of chronic carriers of Salmonella typhi (S. typhosa) first was suggested by Felix et al. (1935). Since that time study of the test by many workers has resulted in the accumulation of a voluminous literature which has been reviewed by Klein (1943), Felix (1951), Landy and Lamb (1953), and Spaun (1957). Bhatnagar et al. (1938) recommended the use ...