Bindin, a protein component of the acrosomal vesicle of sea urchin sperm, has been isolated from Arbacia punctulata and strongylocentrous purpuratus. Using this isolated bindin, we have devised a quantitative assay for bindin-mediated egg agglutination and compared the agglutination of bindin eggs from A. puntulata and S. purpuratus. Bindin- mediated agglutination is species -specific in both s...

Non-specific reactions in agglutination tests have long provided trouble for the bacteriologist. One of the chief offenders has been spontaneous agglutination. The pseudo-reactions obtained in the serologic agglutination of the obligate anaerobes Clostridium paraputrificum (1934) and Clostridium capitovalis (1935), however, were not considered as due to spontaneous agglutination, but rather to ...

AIMS To develop a rapid latex agglutination screening test for invasive amoebiasis. METHODS The performance of an in-house latex agglutination test was compared with three standard serological techniques--the immunofluorescent antibody test (IFAT), the indirect haemagglutination test (IHA), and the cellulose acetate precipitin (CAP) test. Forty six sera were screened; 12 from negative control...

OBJECTIVES To compare the performance of three diagnostic methods for Trichomonas vaginalis infection-latex agglutination, saline wet mount, and culture. METHODS Vaginal swabs from 3807 women attending antenatal clinics were tested for the presence of T vaginalis by latex agglutination. All positives and the following two negatives were tested by wet preparation and culture. RESULTS The pre...

The agglutination of avian red cells, particularly pigeon and budgerigar cells, by sera from patients who had been exposed to birds and had respiratory symptoms, as well as from healthy bird fanciers, has been used to detect the presence of avian specific antibody. Agglutination tests performed by standard techniques using bird erythrocytes can be read within 45 minutes. One hundred and sixty-f...

AIMS To evaluate the Pastorex aspergillus antigen latex agglutination test for the diagnosis of invasive aspergillosis in patients undergoing liver or bone marrow transplantation. METHODS Serum samples were taken at least twice weekly post-transplant and tested for Aspergillus antigen. Latex agglutination test results were compared with microbiological examination of respiratory, urine and bi...

The presence of even a small extent of agglutination among erythrocytes in aqueous suspension can be ascertained by a discrete-particle light-scattering technique. This provides a means of mechanizing a wide variety of assays done in connection with blood-banking, as well as other procedures that rely upon the agglutination of sensitized particles or cells. We describe an agglutination-detectio...

Hybrid, bivalent antibody molecules bearing specific combining sites for both ovalbumin and bovine gamma globulin were produced by reoxidation of a mixture of the 3.5S fragments of the two specifically purified antibodies. The dual specificity and the properties of the hybrid antibody were demonstrated by mixed agglutination and two stage agglutination experiments, and by test systems utilizing...