By KI sI IO AH0 AND CHARLES L. CHRISTIAN I HE TREATMENT of red cells with some proteolytic enzymes has proved to be a suitable technic for the detection of a variety of blood group antibodies which coat but do not agglutinate erythrocytes in physiologic saline. The mechanism of this enzyme effect is not well understood. There is evidence that the antibodies revealed by the enzyme technic are no...

The ability of certain viruses, notably influenza, to agglutinate red blood cells has led to a very useful immunological technique. Certain bacteria also have been reported to possess this capacity by Kraus and Ludwig (1902), Pearce and Winne (1904), Guyot (1908), and Fukuhara (1909). More recently Keogh et al. (1947, 1948) have shown that Hemophilus pertussis, Hemophilus parapertussis, and Hem...

By KI sI IO AH0 AND CHARLES L. CHRISTIAN I HE TREATMENT of red cells with some proteolytic enzymes has proved to be a suitable technic for the detection of a variety of blood group antibodies which coat but do not agglutinate erythrocytes in physiologic saline. The mechanism of this enzyme effect is not well understood. There is evidence that the antibodies revealed by the enzyme technic are no...

Monoclonal antibodies against 12 of the 17 IATS serotype strains of Pseudomonas aeruginosa were produced. Eighty-seven hybridoma clones were isolated, and the antibodies secreted were found to be reactive with both Formalin-fixed whole cells and purified lipopolysaccharide of homologous strains in enzyme-linked immunosorbent assays. Among these monoclonal antibodies, the predominant antibody cl...

Agglutination of red blood cells (RBCs), including chicken RBCs (cRBCs), has been used extensively to estimate viral titer, to screen glycan-receptor binding preference, and to assess the protective response of vaccines. Although this assay enjoys widespread use, some virus strains do not agglutinate RBCs. To address these underlying issues and to increase the usefulness of cRBCs as tools for s...

It has been shown by our experiments that the serum of rabbits treated with emulsions of Treponema pallidum contains agglutinating substances. Normal rabbit serum also possesses agglutinating power for this organism, but, as in the case of normal bacterial agglutinins, to an extent very much inferior to that possessed by the sera of immunized animals. Normal human sera will agglutinate similar ...

Staphylococcus aureus is a prominent bacterial pathogen that is known to agglutinate in the presence of human plasma to form stable clumps. There is increasing evidence that agglutination aids S. aureus pathogenesis, but the mechanisms of this process remain to be fully elucidated. To better define this process, we developed both tube based and flow cytometry methods to monitor clumping in the ...

Pathogenic strains of Entamoeba histolytica are more easily agglutinated with concanavalin A (Con A) than strains isolated from human asymptomatic carriers. All three pathogenic strains studied here were found to agglutinate with low concentrations of Con A in contrast to various nonpathogenic axenic strains of amebas, characterized by their ability to grow at room temperature. Our present obse...

Hybridoma-secreting monoclonal antibodies (MAbs) against colonization factor antigen I (CFA/I) were produced by the fusion of spleen cells from immunized BALB/c mice with F/O myeloma cells. The 25 MAbs with the highest antibody titer against CFA/I, as determined by an enzyme-linked immunosorbent assay, were studied for specificity and the capacity to agglutinate CFA/I-carrying bacteria. Most of...

Précis: Glass composition of individual agglutinate grain is determined largely by the composition of a few soil grains, heterogeneous with respect to the bulk soil, impacted by a micrometeorite. Common perception or consensus: Lunar agglutinates form upon micrometeoritic bombardment of lunar soils and consequent preferential melting of the (otherwise homogeneous) finer fraction, scavenging unm...