BACKGROUND AND AIM: Streptococcus mutans (S. mutans)is a well-known bacterial cause of dental caries. The aim of this study was to develop an herbal mouthwash with anti-biofilm activity and compare it with a commercial preparation containing 2% chlorhexidine. METHODS: The main components of the mouthwashes included Punica...

The antibacterial activity of the root, stem and leaf extracts of Excoecaria agallocha L. was tested against the test organisms Bacillus subtilis, Escherichia coli, Klebsiella pneumoniae, Salmonella spp., Shigella sonnei and Staphylococcus aureus. The activity was tested both by agar well diffusion and disc diffusion methods. It was found that the extracts were very effective in controlling the...

Aim: To evaluate the anti-bacterial potential of pomegranate against Salmonella typhi. Methodology: Ethanolic extracts of pomegranate's different parts; peel, pericarp and fruit were screened for anti-bacterial activity against Multi-Drug Resistance (MDR) strain (UHS-14) by agar well diffusion method. 6% phenol was used as positive control. Results: The peel extract had the largest inhibitory z...

BACKGROUND Disk diffusion susceptibility testing for Leptospira spp. on Leptospira Vanaporn Wuthiekanun (LVW) solid agar was reported recently. However, it was unclear whether the zone sizes obtained on LVW agar were comparable with those of other bacteria on Mueller-Hinton agar. METHODS Here, we evaluate the disk diffusion assay on LVW agar using the standard quality control (QC) bacterial s...

Adhathoda vasica is a well-known herb used in Ayurvedic system of medicine which possesses diverse pharmacological properties. In the present study leaves of Adhathoda vasica were extracted using four different solvents of increasing polarities (chloroform, methanol, ethanol and water). The efficacy of these extracts was tested against various bacterial strains through agar well-diffusion metho...

The E test was evaluated in comparison with reference agar methods (National Committee for Clinical Laboratory Standards) for the susceptibility testing of 248 Pseudomonas aeruginosa isolates from bladder-catheterized patients against nine antibiotics. The E-test MICs correlated well with those determined by the agar dilution and disk diffusion reference methods (88 and 92.5% within 1 log2 dilu...

Although conventional antibiotic susceptibility tests are most commonly performed for methicillin-resistant Staphylococcus aureus (MRSA), the results of these phenotypic tests are dependent on the standardization of the culture conditions. The aim of the study was to evaluate the conventional phenotypic screening tests in comparison to the mecA gene polymerase chain reaction (PCR). One hundred ...