background: cross antigenicity is the major problem in developing a reliable tool for immunodiagnosis and immunoprophylaxis of parasitic diseases. mixed infection due to different types of gastrointestinal parasites is more common than single species infection under field condition. methods: the present study was undertaken to detect antigenic cross-reactivity among haemonchus contortus, oesoph...

Comparative studies of muscle tissue and liver pigs recovered from intracerebral hematoma were carried out using proteomics histology methods. The absence pathological changes in the muscles animals during accumulation proteins with molecular weights 70 to 15 kDa limbs, 50 20 back was established. At same time, destructive structure convalescents revealed masses less than kDa. Thus, it has been...

To study the influence of season on oxidative status of buffalo semen and their association with semen characteristics and seminal plasma proteins, ejaculates were collected twice a week in winter, summer and rainy seasons from six adult Bhadawari buffalo bulls. The neat semen was analyzed for semen characteristics immediately after collection and oxidative status viz. lipid peroxidation (LPO),...

Antibodies are essential tools of biomedical and biochemical researches. Polyclonal antibodies are produced against different epitopes of antigens. Purified F(ab')2 can be used for animal’s immunization to produce polyclonal antibodies. Human immunoglobulin G (IgG) was purified by ion exchange chromatography method. In all stages verification method of the purified antibodies was sod...

Protein is one of the nutrient components in milk that related to product quality. The protein are divided into casein alpha-s1, beta, alpha-s2, kappa, and whey fractions such as alpha lactalbumin beta lactoglobulin. There no existing data fraction dairy cow goats Banyumas Regency. This study aimed determine profile form goat Banyumas. Milk sample from fifty cows thirty was taken by random samp...

background: we have previously reported that a 31 kda protein was screened from the excretory-secretory (es) proteins of tichinella spiralis muscle larvae (ml) by immunoproteomics using early infection sera, and the gene encoding a 31 kda protein from t. spiralis was cloned and expressed in an e. coli expression system. in this study, the recombinant 31 kda antigens were used for detection of a...

The effects of transglutaminase (TGase), reductant, and thermal treatment on the cross-linking white proteins in soft-shell turtle eggs were investigated. Egg denatured by reductant (0.83% 2-mercaptoethanol, 2-ME) pretreatment (95 °C 5 min), then catalyzed TGase (1.0 unit/mL). SDS–PAGE showed that without any pretreatments, three major egg (210 kDa, 115 76 kDa proteins) inferior substrates for ...