Sensory and certain microbial analyses were applied to assess the quality of raw fish sold at a market in Siliguri cityof West Bengal, India. In regular surveys undertaken during June to August 2008, a particular fish species was randomly selected, its source was noted and a sensory analysis, the quality index method (QIM) was applied to assess its quality Raw fish samples were also collected a...

Four Aeromonas strains from clinical and environmental samples differed from known species on the basis of rpoD gene sequence. Multilocus phylogenetic analysis and in silico DNA-DNA hybridization confirmed them as four new species even though their 16S rRNA gene sequence similarity with their closest relatives was >98.7%, as occurred for other Aeromonas spp.

Sixty-three nalidixic acid-resistant Aeromonas sp. isolates were obtained from imported shrimp. Phylogenetic analysis of gyrB sequences indicated that 18 were A. enteropelogenes, 26 were A. caviae, and 19 were A. sobria. Double missense mutations in the quinolone resistance-determining region (QRDR) of gyrA at codon 83 (Ser→Val/Ile) and codon 92 (Leu→Met) coupled with a point mutation of parC a...

The intestinal colonization rate of Aeromonas spp. was determined for 52 cesarean-born Peruvian neonates. Rectal swabs were obtained daily from newborns during their postdelivery hospitalization (mean = 5.5 days), and the gross appearances of their feces (blind determinations) were recorded. Aeromonas spp. were recovered from rectal swabs of 12 of 52 (23.1%) infants during their first week of l...

A total of 50 strains of Aeromonas spp. were isolated from 50 water and fish samples, and identified biochemically and genetically. Biochemical characterization indicated that 20 out of 50 aeromonads were A. veronii, 19 were A. caviae, 9 were A. hydrophila and 2 were A. trota. Molecular identification of isolated aeromonads revealed restriction profile of four species i.e. A. sobria, A. media, ...

A collection of 120 strains isolated from stool specimens collected from humans suffering from gastroenteritis and from environmental samples were analyzed by random amplified polymorphic DNA PCR (RAPD), repetitive extragenic palindromic PCR (REP-PCR), and enterobacterial repetitive intergenic consensus sequence PCR (ERIC-PCR). Species of Aeromonoas hydrophila, A. bestiarum, A. salmonicida, A. ...

An easier assessment model would be helpful for high-throughput screening of Aeromonas virulence. The previous study indicated the potential of Tetrahymena as a permissive model to examine virulence of Aeromonas hydrophila. Here our aim was to assess virulence of Aeromonas spp. using two model hosts, a zebrafish assay and Tetrahymena-Aeromonas co-culture, and to examine whether data from the Te...

Acipenser stellatus is one of the most invaluable species of Sturgeon fishes in the world. The purpose of this study was to identify the resident microbial community from the gastrointestinal tract (GIT) of Acipenser stellatus. One hundred and twenty three fungi were isolated from the GIT of 7 Acipenser stellatus samples including Aspergillus spp (45.5%), Trichoderma spp (8.9%) and Cladophialop...

Sir, The genus Aeromonas comprises 14 species, although its taxonomy has not yet been resolved. The main species considered to be pathogenic in humans are Aeromonas hydrophila, Aeromonas caviae and Aeromonas veronii biotype sobria. These species can cause both gastrointestinal and extraintestinal infectious diseases. Aeromonas gastroenteritis is generally self-limiting and, except in immunocomp...

Aeromonas spp is recognized as pathogenic to humans after consumption of contaminated water and food. In the present investigation, 2,323 rectal swab samples from newborns hospitalized in Rio de Janeiro were evaluated with a view to isolating Aeromonas. The samples were collected and sent to the national reference laboratory for cholera and other bacterial intestinal infections, at the Oswaldo ...