The rapid direct and repetitive somatic embryogenesis in Coffea arabica and C. canephora genotypes was tested on Murashige and Skoog medium (MS) containing thidiazuron (TDZ) (1-phenyl-3-(1,2,3,-thiadiazol-5-yl)urea) in concentrations of 2.27–11.35s M. Segments taken from cotyledon leaf, first leaf and stalk of regenerated plantlets produced clusters of somatic embryos directly from cut portions...

Polyploid plantlets, including triploid, tetraploid, and mixoploid, were induced from the European pear (Pyrus communis L.) cultivar ‘Fertility’ by in vitro colchicine treatment of leaf explants. The leaf explants were incubated in 0.4% (w/v) colchicine for 24, 48, or 72 h, then transferred to adventitious shoot-induction medium. Regenerated shoots were pre-selected according to their morpholog...

During post-embryonic development, roots can be initiated by a programmed developmental order or by environmental and wound stimulation (Bellini et al., 2014; Xu and Huang, 2014; Birnbaum, 2016; Ikeuchi et al., 2016; Kareem et al., 2016; Lup et al., 2016; Rellan-Alvarez et al., 2016; Steffens and Rasmussen, 2016). De novo root regeneration (DNRR) is a type of plant regeneration to produce adven...

Different sugarcane varieties (CP 77/400, CP 85/1491 and Punjabi) were checked for its callus induction and regeneration capabilities using different growth regulators and explants (eye bud, upper portion of inner leaf whorls and lower portion of inner leaf whorls). Seven callus induction media i.e. CIM-1 to CIM-6 and control media, five shoot induction media i.e. SIM-1 to SIM-4 and control med...

The genus of Helichrysum comprises many species which have therapeutical effects and used in folk medicine. H. pallasii is one these species, the region for traditional medicine ornamental purposes. Therefore, optimizing alternative micropropagation protocols tissue culture conditions secondary metabolite production needs attention. effect Thidiazuran (TDZ) combined with Zeatin (ZEA) on shoot r...

Micropropagation techniques have been widely applied in globe artichoke (C. cardunculus L. var. scolymus), however, efficient protocols for the establishment of in vitro callogenesis and organogenesis, a pre-requisite for Agrobacteriummediated genetic transformation, have not been set up so far. We developed an efficient protocol for callus induction from leaf explants of three globe artichoke ...

tissue culture methods provide tools to supplement traditional methods for collection, propagation and preservation of endangered plant species. in this study, in vitro propagation of diaphanoptera khorasanica rech.f., a rare and threatened plant species with limited distribution range and population was investigated. this species has a potential as an ornamental plant. single node explants wer...