نتایج جستجو برای: adna

تعداد نتایج: 215  

Journal: :Memórias do Instituto Oswaldo Cruz 2006

2002
Henryk W. Witas

The revolution which introduced new techniques of molecular biology applied to DNA analysis enormously accelerated the progress in most areas of medicine and biology. Techniques such as polymerase chain reaction (PCR), restriction analysis and sequencing are widely used for diagnosis of a number of diseases, for genetic screening, phylogenetic analysis and population studies. Moreover, it becam...

2001
Igor Ovchinnikov William Goodwin

Over the past ten years the analysis of mitochondrial DNA (mtDNA) has become widespread when dealing with very low quantities of and/or highly degraded DNA. The advantages of mtDNA for this type of analysis have been well documented including its high copy number, which improves the chance of retrieving DNA, and its highly polymorphic, and therefore informative nature. In addition to forensic s...

2018
Ashot Margaryan Henrik B Hansen Simon Rasmussen Martin Sikora Vyacheslav Moiseyev Alexandr Khoklov Andrey Epimakhov Levon Yepiskoposyan Aivar Kriiska Liivi Varul Lehti Saag Niels Lynnerup Eske Willerslev Morten E Allentoft

Recent ancient DNA (aDNA) studies of human pathogens have provided invaluable insights into their evolutionary history and prevalence in space and time. Most of these studies were based on DNA extracted from teeth or postcranial bones. In contrast, no pathogen DNA has been reported from the petrous bone which has become the most desired skeletal element in ancient DNA research due to its high e...

Journal: :Human heredity 2013
Marie-France Deguilloux Fanny Mendisco

OBJECTIVES The history of European populations is characterised by numerous migrations or demographic events that are likely to have had major impacts on the European gene pool patterns. This paper will focus on how ancient DNA (aDNA) data contribute to our understanding of past population dynamics in Europe. METHODS Technological challenges of the palaeogenetic approach will be discussed. Wi...

Journal: :American journal of human genetics 2013
Meredith L Carpenter Jason D Buenrostro Cristina Valdiosera Hannes Schroeder Morten E Allentoft Martin Sikora Morten Rasmussen Simon Gravel Sonia Guillén Georgi Nekhrizov Krasimir Leshtakov Diana Dimitrova Nikola Theodossiev Davide Pettener Donata Luiselli Karla Sandoval Andrés Moreno-Estrada Yingrui Li Jun Wang M Thomas P Gilbert Eske Willerslev William J Greenleaf Carlos D Bustamante

Most ancient specimens contain very low levels of endogenous DNA, precluding the shotgun sequencing of many interesting samples because of cost. Ancient DNA (aDNA) libraries often contain <1% endogenous DNA, with the majority of sequencing capacity taken up by environmental DNA. Here we present a capture-based method for enriching the endogenous component of aDNA sequencing libraries. By using ...

2016
Karl-Göran Sjögren T Douglas Price Kristian Kristiansen

Isotopic investigations of two cemetery populations from the Corded Ware Culture in southern Germany reveal new information on the dating of these graves, human diet during this period, and individual mobility. Corded Ware Culture was present across much of temperate Europe ca. 2800-2200 cal. BC and is represented by distinctive artifacts and burial practices. Corded Ware was strongly influence...

2016
Nina Luhmann Daniel Doerr Cedric Chauve

Yersinia pestis is the causative agent of the bubonic plague, a disease responsible for 9 several dramatic historical pandemics. Progress in ancient DNA (aDNA) sequencing rendered possible 10 the sequencing of whole genomes of important human pathogens, including the ancient Yersinia pestis 11 strains responsible for important outbreaks of the bubonic plague in London in the 14th century and 12...

Journal: :Molecular biology and evolution 2007
Simon Y W Ho Tim H Heupink Andrew Rambaut Beth Shapiro

DNA extracted from archaeological and paleontological remains is usually damaged by biochemical processes postmortem. Some of these processes lead to changes in the structure of the DNA molecule, which can result in the incorporation of incorrect nucleotides during polymerase chain reaction. These base misincorporations, or miscoding lesions, can lead to the inclusion of spurious additional mut...

2014
Lauren Hubert Jaeger Alena Mayo Iñiguez

Paleoparasitology is the science that uses parasitological techniques for diagnosing parasitic diseases in the past. Advances in molecular biology brought new insights into this field allowing the study of archaeological material. However, due to technical limitations a proper diagnosis and confirmation of the presence of parasites is not always possible, especially in scarce and degraded archa...

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