The synthesis of the negative-strand [(-)-strand] complement of the ∼30 kilobase, positive-strand [(+)-strand] coronaviral genome is a necessary early step for genome replication. The identification of cis-acting elements required for (-)-strand RNA synthesis in coronaviruses, however, has been hampered due to insufficiencies in the techniques used to detect the (-)-strand RNA species. Here, we...

Agility of the supply chain is one of the main challenges in organizations which acting in a dynamic and complex environment, which they have to be able to answer to customers quickly. But the agility of supply chain is influenced by different agents. Using the model based on agent, this study has tried to simulate the agility of supply chain in an institute of higher education. There are three...

Adenosine-to-inosine RNA editing modifies maturing mRNAs through the binding of adenosine deaminase acting on RNA (Adar) proteins to double-stranded RNA structures in a process critical for neuronal function. Editing levels at individual editing sites span a broad range and are mediated by both cis-acting elements (surrounding RNA sequence and secondary structure) and trans-acting factors. Here...

ADP-glucose pyrophosphorylase (AGPase) is a key regulatory enzyme for starch synthesis in plant. Starch is an important carbohydrate consisting of a large number of glucose units and the primary energy source in plants. The essential transcriptional gene regulatory units are cis-acting regulatory elements as they control various stress responses. Therefore, understanding of the transcriptional ...

Pathogen-inducible plant promoters contain multiple cis-acting elements, only some of which may contribute to pathogen inducibility. Therefore, we made defined synthetic promoters containing tetramers of only a single type of element and present evidence that a range of cis-acting elements (boxes W1, W2, GCC, JERE, S, Gst1, and D) can mediate local gene expression in planta after pathogen attac...