are heterogeneous with respect to molecular weight, buoyant density, and thermal stability. This finding constitutes evidence against unique points of breakage during DNA isolation. The variation in buoyant density suggests a local compositional heterogeneity in the chromosomal region of certain markers. The variation in molecular weight provides an explanation for the results of certain transf...

Two structurally similar compounds were found to inhibit sporulation in Bacillus subtilis 168. A dye, acridine orange, and an antischizophrenic drug, promethazine, blocked spore formation at concentrations subinhibitory to vegetative growth, while allowing synthesis of serine protease, antibiotic, and certain catabolite-repressed enzymes. The sporulation process was sensitive to promethazine th...

with Acridine Orange By DAvID M. JOHNSON, PEYTON T. PIiArr AND PEnnY G. RuGBY I HE IMPORTANCE OF lymphocytic RNA in immune function has received widespread attention in recent years.’ A case in point is the work of Mannick and Egdahl showing that adoptive immunity can be transferred from one animal to another by means of lymphocytic RNA.2 The RNA of the lymphocyte can be evaluated and compared ...

The effects of odor exposure during the last 2 days of incubation and immediately after hatching on subsequent responses by chicks to that same scent were assessed. When tested with an orange-treated and an unscented container, chicks that had been previously exposed to orange odor more rapidly approached both containers than did control chicks and spent more time near them. In both conditions,...

Three-part differential white blood cell counts are used for disease diagnosis and monitoring at the point-of-care. A low-cost, miniature achromatic microscope was fabricated for identification of lymphocytes, monocytes, and granulocytes in samples of whole blood stained with acridine orange. The microscope was manufactured using rapid prototyping techniques of diamond turning and 3D printing a...