Serum-free cultured neuroblastoma cells (clone NlE-115) have been shown to absorb emulsified glucosylceramide, glucosylceramide glucosidase, an activator protein for the enzyme, and phosphatidylserine from a synthetic medium. Uptake of the enzyme was augmented by phosphatidylserine, and vice versa. Uptake of the enzyme-lipid complex was further augmented by the activator protein. It appears lik...

The kinetics of oxidation of semicarbazide by 12-tungstocobaltate (III) in aquous acidic medium at 300K and a constant ionic strength of 0.3 mol dm was studied spectrophotometrically at λmax 624nm under pseudo first-order conditions. The reaction between semicarbazide and 12-tungstocobaltate (III) in acidic medium exhibits 1:2 stiochiometry [Semicarbazide:12-tungstocobaltate (III)]. The main ox...

The supramolecular architecture of the basement membrane is provided by two enmeshed networks of collagen IV and laminin. The laminin network is maintained exclusively by interactions among individual laminin molecules and does not depend on the presence of other extracellular matrix components. Laminin polymers can be obtained in vitro either in solution or in association with the surface of b...

Agrobacterium tumefaciens C58 grown on acidic medium containing glucose and solidified with bacteriological agar expressed a virB::lacZ fusion. No expression of this fusion was observed on a similar medium which was solidified with purified agarose. The fraction from bacteriological agar which was responsible for vir gene induction was extracted with methanol and partially purified by preparati...