OBJECTIVE Increased inflammatory response may be associated with adverse clinical outcomes, especially in the neonatal period. The aims of this study were to determine whether N-acetyl-cysteine (NAC), an anti-inflammatory agent, attenuates the inflammatory response in young rats and to determine the most effective route of administration. METHODS Four groups of Sprague-Dawley rats (in each gr...

In this study, we evaluated the effect of lipoic acid (LA) and N-acetyl cysteine (NAC) on oxidative [4-hydroxy-2-nonenal, N(epsilon)-(carboxymethyl)lysine and heme oxygenase-1] and apoptotic (caspase 9 and Bax) markers in fibroblasts from patients with Alzheimer disease (AD) and age-matched and young controls. AD fibroblasts showed the highest levels of oxidative stress, and the antioxidants, l...

Abstract: Seeds of the genus Eugenia L. (Myrtaceae) have interesting propagation strategies, and reactive oxygen species (ROS) seem to be related their germination seedling production. In this study, effects ROS on E. involucrata seeds were analyzed indirectly, with application N-acetyl-L-cysteine (NAC), an antioxidant. The results demonstrated that incubation in NAC suppressed and/or delayed s...

Swertia mileensis, known as Qing-Ye-Dan (QYD), has been documented in Chinese Pharmacopoeia to cure hepatitis. Interestingly, its announced main active component, swertiamarin, could not be detected the decoction, which indicated that efficacy of QYD might attributed heat-transformed products swertiamarin (HTPS). Further investigation on HTPS led isolation sweritranslactone D (1), a novel secoi...

The structure and reactivity of the N-acetyl-cysteine radical cation and anion were studied using ion-molecule reactions, infrared multi-photon dissociation (IRMPD) spectroscopy, and density functional theory (DFT) calculations. The radical cation was generated by first nitrosylating the thiol of N-acetyl-cysteine followed by the homolytic cleavage of the S-NO bond in the gas phase. IRMPD spect...

A UHPLC-(ESI+)-MS/MS method was developed and validated for the simultaneous determination of glucoraphanin (GR), sulforaphane (SF), sulforaphane-glutathione (SF-GSH), sulforaphane-cysteine (SF-CYS), sulforaphane-N-acetyl-cysteine (SF-NAC), in a range biological materials (digesta, cell culture medium, lysates, urine) with no sample pretreatment. The limit detection (LODs) ranged from 0.03 to 1...