A particulate enzyme preparation prepared from the intimal layer of pig aorta catalyzed the transfer of mannose from mannosyl-phosphoryl-polyprenol (MPP) into a series of oligosaccharides that were linked to lipid. The reaction required detergent with Triton X-100 and NP-40 being best at a concentration of 0.5%. Several other detergents were inactive or only slightly active. The pH optima for t...

A complete molecular structure for an antigenic glycan isolated from the cell wall of Streptococcus faecalis is being proposed on the basis of analytical data obtained from methylation, acetolysis, and periodate oxidation experiments and on the basis of the nature of the oligosaccharide fragments isolated from partial acid hydrolysates of the glycan. The antigen is a diheteroglycan composed of ...

Manumycin (1), produced by Streptomyces parvulus (strain Tü 64), was isolated from the mycelium by extraction with acetone and could easily be purified chromatographically. Chemical degradation of 1 (C31H38N2O7) gave 2-acetamino-3-hydroxycyclopent-2-enone (2) by acetolysis, 2,4,6-trimethyl-2,4-decadienoic acid (3) by alkaline hydrolysis, and 2-(2,4,6-trimethyl-2,4-decadienoylamino)-5,6-epoxy-1,...

We previously reported the isolation of two cerulenin-resistant mutant strains of Candida albicans 4918 that differ in adherence properties and are less virulent than the parental strain. In addition, biochemical characterization demonstrated significant differences in both protein and polysaccharide composition of cell wall material between the mutant and wild-type strains. These observations ...

To investigate the chemical structure of cell wall mannan of pathogenic yeast, Candida catenulata IFO 0745 strain, which possess the epitopes of antigenic factors 1, 9, and 34 to genus Candida, we previously performed the two-dimensional nuclear magnetic resonance (NMR) analysis of this mannan, Fr. C, without the need for harsh procedures. In this study, three oligosaccharides, biose, triose, a...