The effects of different proportions La and Y elements in the A-side on structure properties A2B7-type La–Y–Ni hydrogen storage alloys were investigated. (La,Y)2Ni7 with La/Y ratios prepared by sintering Y2Ni4 precursor AB5-type precursors at 1298 K for 5 h subsequently annealed 20 1248 K. All only contain Ce2Ni7 (2H-type) Gd2Co7 (3R-type) phases mass ratios. As ratio decreases, cell volume two...

Japanese encephalitis virus (JEV) is an enveloped icosahedral capsid with a prime neutralizing epitope present in E protein domain III (EDIII). dimers are rearranged into five-fold symmetry of icosahedrons. Cholera toxin B (CTB) and heat-labile enterotoxin (LTB) AB5-type was used as the structural scaffold for emulating pentameric axis EDIII. We produced homo-pentameric EDIII through genetic fu...

The subcellular localization and corresponding quaternary state of fluorescent labelled cholera toxin were determined at different time points after exposure to living cells by a novel form of fluorescence confocal microscopy. The compartmentalization and locus of separation of the pentameric B subunits (CTB) from the A subunit (CTA) of the toxin were evaluated on a pixel-by-pixel (voxel-by-vox...

The Shiga toxigenic Escherichia coli (STEC) O113:H21 strain 98NK2, which was responsible for an outbreak of hemolytic uremic syndrome, secretes a highly potent and lethal subtilase cytotoxin that is unrelated to any bacterial toxin described to date. It is the prototype of a new family of AB(5) toxins, comprising a single 35-kilodalton (kD) A subunit and a pentamer of 13-kD B subunits. The A su...