BACKGROUND Reactive oxygen species (ROS) have been implicated in various forms of cellular injury. ROS may cause cell damage and are involved in the pathophysiology of several diseases, including atherosclerosis and chronic inflammation. METHODS Disturbances of intracellular ROS levels were investigated in 28 patients with end-stage renal failure. The intracellular ROS levels were measured in...

HESSELTINE, C. W. (Northern Regional Research Laboratory, Peoria, Ill.), AND K. SHIBASAKI. Miso. III. Pure culture fermentation with Saccharomyces rouxii. Appl. Microbiol. 9:515-518. 1961.-Excellent miso has been prepared with soybean grits inoculated with a pure culture of Saccharomyces rouxii strain NRRL Y-2547. Pure culture inoculum of this osmophilic yeast was prepared by growing the cultur...

A51-year-old adopted white female was admitted to the intensive care unit with hepatic encephalopathy secondary to an upper gastrointestinal hemorrhage on a background of alcohol-induced chronic liver disease. She was hemodynamically stable (blood pressure 119/64 mm Hg) but pyrexial (39.0°C) and in atrial fibrillation, with a poorly controlled ventricular rate (Figure 1). Her regular medication...

Genome-wide DNA remodelling in the ciliate Paramecium is ensured by RNA-mediated trans-nuclear crosstalk between the germline and the somatic genomes during sexual development. The rearrangements include elimination of transposable elements, minisatellites and tens of thousands non-coding elements called internally eliminated sequences (IESs). The trans-nuclear genome comparison process employs...

In many eukaryotes, RNA-dependent RNA polymerases (RdRPs) play key roles in the RNAi pathway. They have been implicated in the recognition and processing of aberrant transcripts triggering the process, and in amplification of the silencing response. We have tested the functions of RdRP genes from the ciliate Paramecium tetraurelia in experimentally induced and endogenous mechanisms of gene sile...

A detailed theoretical study of magnetic behavior of iron along the bcc-fcc (Bain’s) transformation paths at various atomic volumes, using both the local spin-density approximation (LSDA) and the generalized gradient approximation (GGA), is presented. The total energies are calculated by the spin-polarized fullpotential linearized augmented plane waves method and are displayed in contour plots ...

UNLABELLED Antigenic or phenotypic variation is a widespread phenomenon of expression of variable surface protein coats on eukaryotic microbes. To clarify the mechanism behind mutually exclusive gene expression, we characterized the genetic properties of the surface antigen multigene family in the ciliate Paramecium tetraurelia and the epigenetic factors controlling expression and silencing. Ge...

SNARE proteins (soluble N-ethylmaleimide-sensitive factor attachment protein receptors) mediate membrane interactions and are conventionally divided into Q-SNAREs and R-SNAREs according to the possession of a glutamine or arginine residue at the core of their SNARE domain. Here, we describe a set of R-SNAREs from the ciliate Paramecium tetraurelia consisting of seven families encoded by 12 gene...

Tb-Yb co-doped NaYF4 nanoparticles (NPs) are prepared by sintering the assynthesized NaYF4:Tb, Yb NPs at 380°C under air atmosphere. The oxidization of Tb ions to Tb ions in NaYF4 NPs after sintering is demonstrated through X-ray photoelectron spectroscopy (XPS). The near-infrared (NIR) downconversion (DC) luminescence of TbYb couple is measured and investigated for the first time. The results ...

Holas and March (Phys. Rev. A51, 2040, 1995) gave a formally exact expression for the force @Vxc(r ~ ) @r ~ associated with the exchange-correlation potential Vxc(r ~ ) of density functional theory. This forged a precise link between rstand second-order density matrices and Vxc(r ~ ). Here models are presented in which these low-order matrices can be related to the ground-state electron density...