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Liver fibrosis assessment is very important to the treatment of chronic liver disease. In the present study, Virtual Touch Tissue Quantification (VTQ) and eSie Touch™ elasticity imaging techniques were used to examine the rat liver fibrosis model. Rat liver fibrosis was induced with thioacetamide and the degree of liver fibrosis was determined using pathological diagnosis as a gold standard. Th...
Influence of ionic strength on the kinetics of the promoter complex formation between E. coli RNA polymerase and T7 phage DNA was investigated using a membrane filter assay. The enzyme-promoter association rate constant was determined. It varies from 10(9) to 3 x 10(7) M-1 sec-1 when the ionic strength is changed from zero to 0.15 M NaCl. Basing on the theoretical analysis of experimental data ...
Selenocysteine-incorporating tRNA(Sec)(UCA), the product of selC, was isolated from E.coli and aminoacylated with serine. The equilibrium dissociation constant for the interaction of Ser-tRNA(Sec)(UCA) with elongation factor Tu.GTP was determined to be 5.0 +/- 2.5 x 10(-8) M. Compared with the dissociation constants of the two elongator Ser-tRNA(Ser) species (Kd = 7 x 10(-10) M), the selenocyst...
Isothermal titration calorimetry (ITC) is now routinely used to directly characterize the thermodynamics of biopolymer binding interactions and the kinetics of enzyme-catalyzed reactions. This is the result of improvements in ITC instrumentation and data analysis software. Modern ITC instruments make it possible to measure heat effects as small as 0.1 microcal (0.4 microJ), allowing the determi...
The physical properties of the synthesized MAX phases M 2 SeC (M = Hf, Zr) have been investigated for first time using DFT method in pressure range 0–25 GPa. computed lattice parameters are consistent with earlier findings at ambient pressure. electronic band structure and energy dependent density states reveal metallic nature titled compounds. behavior remains unchanged within studied range. P...
In semi-arid regions, soils are weakly aggregated and subjected to water erosion processes especially rill and interrill erosion. There is no information on the rate of these water erosion types in semi-arid soils located in the hillslopes. Therefore, this study was conducted to determine the soils susceptibility to these erosion types in semi-arid region. A laboratory experiment was done in ei...
Abstract Pedestrians can cross the road safely if suitable facilities are provided for traffic conditions. The purpose of this study is to analyze characteristics pedestrians at zebra crossing and choose alternative confirm safety environmentally friendly pedestrian facilities. Based on analysis, men crossed faster than women, men’s speed was 1.28 m/sec women’s 1.20 m/sec. individual platoon, 1...
Recently, the indication for transcatheter aortic valve replacement (TAVR) has been expanded not only to patients with degenerative aortic stenosis considered inoperable or of high surgical risk but also to those with intermediate surgical risk. Thus, optimal patient risk stratification should be performed before the TAVR procedure. Frailty, which is not captured in the classical surgical risk ...
a study was carried out to map dispersal of trichogramma brassicae bezd. on tomato in a 0.5 ha field. first, 1, 5, 10, 15, 20 and 25 meter distances from the central point of the field were marked in form of squares. on each square, 8 points were selected and a wooden stake of 1.5 m length was vertically secured into the soil at each point equal to plant height and bearing a yellow sticky trap ...
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