A system for detecting the effects of metal ions, repressors, and mutations on the biosynthesis in vivo of alkaline phosphatase of Escherichia coli is described. If E. coli constitutive for alkaline phosphatase are grown in a medium containing 65Zn and are subject to osmotic shock, about 50% of the zinc taken up by the cells is released into the medium. Chromatography of this medium shows that ...

The production cross-section of 68Ge, 69Ge, 65Zn and 67Ga radioisotopes from alpha-induced nuclear reaction with natZn have been measured using the stacked foil activation technique followed by off-line γ-ray spectroscopy in incident alpha energy range 14-37 MeV. obtained cross-sections are compared previous experimental data available EXFOR library, evaluated TENDL-2019 theoretical results, ca...

Starved cells of Candida utilis accumulated Zn2+ by two different processes. The first was a rapid, energy- and temperature-independent system that probably represented binding to the cell surface. The cells also possessed an energy-, pH-, and temperature-dependent system that was capable of accumulating much greater quantities of the cation than the binding process. The energy-dependent system...

By Energy Dispersive X-ray fluorescence we have determined that calf thymus poly(ADP-ribose) polymerase binds two zinc ions per enzyme molecule. Using 65Zn (II) for detection of zinc binding proteins and polypeptides on western blots, we found that the zinc binding sites are localized in a 29 kd N-terminal fragment which is included in the DNA binding domain. Metal depletion and restoration exp...

Spermatidal transition protein, TP2, was purified from rat testes by Hg-affinity chromatography. The present study reports the details of the zinc-metalloprotein nature of TP2 by employing the 65 Zn-blotting technique. Chemical modification of cysteine by iodoacetic acid, and histidine by diethylpyrocarbonate, resulted in a near complete inhibition of 65Zn-binding to TP2. The 65Zinc-binding was...

The binding of five biologically important metals to serum proteins has been studied. After suitable radioactive isotopes were added to serum proteins separated and precipitated by two-dimensional immunoelectrophoresis, the sample plates were exposed to roentgenogram film. 59Fe bound to transferrin alone; 65Zn bound mostly to albumin, but also to another 12 proteins; 109Cd was mostly associated...