Structural and mechanistic characterization of proteins by fluorescence resonance energy transfer (FRET)1,2 requires the ability to incorporate fluorescent probes at specific, defined sites.2 For proteins that do not contain cysteine residues, site-specific fluorescent labeling can be accomplished by use of site-directed mutagenesis to introduce a cysteine residue at the site of interest, follo...

Triple-negative breast cancers (TNBC),which include the basallike and claudin-low disease subtypes, are aggressive malignancies for which effective therapeutic targets are lacking. NF-kB activation has an established role in breast malignancy, and it is higher in TNBC than other breast cancer subtypes. On this basis, we hypothesized that proteins derived from NF-kB target genes might be molecul...

We tested the hypothesis of synchronous interannual changes in forage fish dynamics around the North Pacific Rim. To do this, we sampled forage fish communities using a seabird predator, the rhinoceros auklet (Cerorhinca monocerata), at six coastal study sites from Japan to California. We investigated whether take of forage fishes was related to local marine conditions as indexed by sea surface...

Triple-negative breast cancers (TNBC),which include the basallike and claudin-low disease subtypes, are aggressive malignancies for which effective therapeutic targets are lacking. NF-kB activation has an established role in breast malignancy, and it is higher in TNBC than other breast cancer subtypes. On this basis, we hypothesized that proteins derived from NF-kB target genes might be molecul...

Carotid bifurcation stenosis may coexist simultaneously with more proximal common carotid artery (CCA) atherosclerotic plaquing, primarily at the vessel origin in aortic arch. Obtaining long-term outcomes data is vital to making any definitive conclusions on efficacy of performing this dual treatment. Four cohorts were created for perioperative outcome analysis and Kaplan-Meier freedom from eve...

Mutualistic microbes associated with plants have enormous potential for economical and sustainable agriculture.There are two approaches to plant microbiome engineering: the bottom-up approach that involves isolating, engineering, reintroducing specific microbes, top-down synthetic ecology, using horizontal gene transfer a broad range of hosts in situ then phenotyping microbiome.Recent advances ...

24 Identification of a novel class of anti-Burkholderia compounds is key in 25 addressing antimicrobial resistance to current therapies as well as naturally 26 occurring resistance. The FabI enoyl-ACP reductase in Burkholderia is an 27 underexploited target that presents an opportunity for development of a new 28 class of inhibitors. A library of substituted diphenyl ethers were used to identif...