Rose is the most favorite cut flowers all over the world. Production of high-quality flowers, prevention, and delay of flower senescence, is a major goal in floriculture. Now a day, biotechnological approaches have been used to improve ornamental attributes. Tissue culture and genetic transformation appear to offer valuable advancements for operating floral characteristics. In this study, after...

Shoot-tip cultures of Pyrus elaeagrifolia Pallas, an important gene source for drought and chlorosis resistance in pear rootstock breeding, were established from a wild mature tree originated from seed. Murashige and Skoog basal medium supplemented with different concentrations of benzyladenine (BA) singly or in combination with auxin was used in the study. In the initial culture, the highest p...

An efficient in vitro plant regeneration protocol was developed for Chrysanthemum morifolium, an ornamental plant. Nodal segments were used as explants in the present experiment. The explants were cultured onto Murashige & Skoog’s (MS) nutrient medium containing plant growth regulators (cytokinins or auxins) with various combinations and concentrations for the study. Maximum percentage (93.33%)...

introduction: potential almond rootstock and thus main genetic sources for the breeding of its new rootstocks are almond itself, peach and plum, and these species cross-breeds almond is a source of resistance to limestone and to drought because of its roots, which reach deep layers of soil; it is also more resistant than peach to na and bo. asexual or vegetative propagation is a hereditable cha...

A protocol was developed for high frequency in vitro multiplication of an ornamental orchid, Phalaenopsis amabilis (L.) Blume var. Jawa, using plant growth regulators (PGRs). Protocorm-like bodies (PLBs), as explants were cultured on Murashige and Skoog (MS) medium fortified with various concentrations of kinetin (KIN) and indole-3-butyric acid (IBA), either individually or in...

Production of genetically and phenotypically stable plantlets is the main purpose in commercial strawberry tissue culture. In this study, different tissues of Fragaria ananassa cv. Camarosa including stipule, apical meristem, leaf and petiole were cultured in Murashige and Skoog (MS) medium supplemented withdifferent concentrations of N6-benzyladenine (BA) (0.5, 1, ...

Hairy root culture is a promising approach to improve production of plant secondary metabolites. The genes, which are located in T-DNA root-inducing plasmid, regulate auxin sensitivity hairy roots. Therefore, this study was aimed the growth and rosmarinic acid Lavandula angustifolia Lateral branches roots were transferred ½ MS B5 liquid media. To assess sensitivity, indole-3-acetic (IAA) indole...

An efficient in vitro protocol was standardized for Almond (Prunus dulcis) propagation using dormant axillary buds as explants. Explants were cultured on Murashige and Skoog (MS) and woody plant medium (WPM) supplemented with different concentration/combination(s) of phytohormones. MS basal medium showed lowest shoot induction and took longest duration for shoot initiation. Multiple shoots were...

Valerian (Valeriana officinalis L.) is an important medicinal plant that is widely exploited for its roots and rhizomes which contain valepotriates and valerenic acids (with putative pharmacological activities). Thus root proliferation of valerian is very important. The aim of this study was to establish a practical tissue culture method for rapid and large-scale induction of V. officinalis L. ...

Leaf and stem explants excised from young plant of B.diffusa  were cultured on Murashige and Skoog (MS) medium containing agar (0.8%), sucrose (2.5%) and varied concentration of Indole butyric acid (IBA), Napthyl acetic acid (NAA), 2,4 - dichloro phenoxy acetic acid (2,4-D) and Picrolam. Leaf explants has given better response for both rooting and callus formation. IBA in a concentration of 5μM...