نتایج جستجو برای: 30 expression vector

تعداد نتایج: 1528184  

Journal: :jundishapur journal of microbiology 0
mojtaba fattahi abdizadeh infectious and tropical diseases research center, ahvaz jundishapur university of medical sciences, ahvaz, ir iran; department of virology, institute pasteur of iran, tehran, ir iran manoochehr makvandi infectious and tropical diseases research center, ahvaz jundishapur university of medical sciences, ahvaz, ir iran; department of virology, ahvaz jundishapur university of medical sciences, ahvaz, ir iran; infectious and tropical diseases research center, ahvaz jundishapur university of medical sciences, ahvaz, ir iran. tel: +98-6113738313, fax: +98-6113738313 alireza samarbafzadeh department of virology, ahvaz jundishapur university of medical sciences, ahvaz, ir iran kayhan azadmanesh department of virology, institute pasteur of iran, tehran, ir iran

conclusions according to the high functionality of the produced recombinant vector, it seems a good applicable tool for making an indicator cell line in subsequent basic and drug studies. results recombinant vector, pgl4ltr-luc, has expression levels of more than 50 folds compared to the control when co-transfected with the tax expressing vector into hek 293t cells. moreover, cells display more...

Journal: :iranian journal of microbiology 0
parastoo aziminia islamic azad university, karaj branch, department of microbiology, karaj, iran reza pilehchian langroudi clostridia specialized research laboratory, razi vaccine and serum research institute, agricultural research, education and extension organization (areeo), karaj, iran kasra esmaeilnia razi vaccine and serum research institute, agricultural research, education and extension organization (areeo), karaj, iran

background and objectives: clostridium perfringens, a gram-positive obligate anaerobic bacterium, is able to form resistant spores which are widely distributed in the environment. c. perfringens is subdivided into five types a to e based on its four major alpha, beta, epsilon and iota toxins. the aim of the present study was cloning and expression of c. perfringens type d vaccine strain epsilon...

Journal: :journal of sciences islamic republic of iran 0

we studied the expression of human growth hormone (hgh) in e. coli under a bacteriophage t5-base promoter in a pqe30 expression vector. for an efficient expression of hgh cdna, a number of codons at the hgh n-terminal coding region were altered based on the e. coli major codons. an over-expression of hgh in the bacteria, carrying the recombinant plasmids, was observed at 37°c in the presence of...

Journal: :iranian journal of allergy, asthma and immunology 0
parvin mosadeghi department of biology, basic science faculty, payam noor university of mashhad, mashhad, iran hafez heydari zarnagh molecular and cellular research center, sabzevar university of medical science, sabzevar, iran mohammad mohammad-zadeh molecular and cellular research center, sabzevar university of medical science, sabzevar, iran masoud salehi moghaddam department of biology, basic science faculty, payam noor university of mashhad, mashhad, iran

expression of htlv-i p19 protein in an escherichia coli expression system always leads to the formation of inclusion body. solubilisation and refolding of the inclusion bodies is complex, time consuming and difficult during large-scale preparation. this study aimed to express and purify a soluble form of recombinant htlv-i p19 protein in an e. coli expression system. the synthetic dna encoding ...

Journal: :journal of artificial intelligence in electrical engineering 2016
saeede jabbarzadeh reyhani saeed meshgini

classical lbp such as complexity and high dimensions of feature vectors that make it necessary to apply dimension reduction processes. in this paper, we introduce an improved lbp algorithm to solve these problems that utilizes fast pca algorithm for reduction of vector dimensions of extracted features. in other words, proffer method (fast pca+lbp) is an improved lbp algorithm that is extracted ...

ژورنال: دانشور پزشکی 2007
جورابچی, عصمت , حقیقی, هاله, قربانلی, مه لقا, هاشمی سهی, هاله,

Introduction: Human growth hormone (HGH) is very important pharmaceutical substance which is used as an absolute cure for different diseases that is why the production of HGH recombinant is taken into consideration not only nationally but also internationally. Many different methods and host systems have been used as growth hormone factories. Finding new and efficient hosts for recombinant mole...

We studied the expression of human growth hormone (hGH) in E. coli under a bacteriophage T5-base promoter in a pQE30 expression vector. For an efficient expression of hGH cDNA, a number of codons at the hGH N-terminal coding region were altered based on the E. coli major codons. An over-expression of hGH in the bacteria, carrying the recombinant plasmids, was observed at 37°C in the presence of...

Journal: :iranian journal of allergy, asthma and immunology 0
fatemeh dadgar pakdel department of medical immunology, school of medicine, tehran university of medical sciences tehran, iran and department of medical genetics, school of medicine, tehran university of medical sciences, tehran, iran . mohammad keramatipour department of medical genetics, school of medicine, tehran university of medical sciences, tehran, iran farshid noorbakhsh department of medical immunology, school of medicine, tehran university of medical sciences, tehran, iran saeed talebi department of medical genetics, school of medicine, tehran university of medical sciences, tehran, iran mohammad vodjgani department of medical immunology, school of medicine, tehran university of medical sciences, tehran, iran

the interaction between immune cells and endothelial lining of blood vessels is vital in many processes such as inflammatory and immune responses as well as cancer cell metastasis. the expression level of vcam-1 is regulated by many factors including the promoter activity that is possibly affected by the single nucleotide polymorphisms (snps) present in the promoter. there are previous reports ...

ژورنال: دانشور پزشکی 2009

 Introduction: RNA interference (RNAi) is a phenomenon of gene silencing that uses double-stranded RNA (dsRNA), specifically inhibits gene expression by degrading mRNA efficiently. The mediators of degradation are 21- to 23-nt small interfering RNAs (siRNA). The use of siRNAs as inhibitors of gene expression has been shown to be an effective way of studying gene function in mammalian cells.  Ai...

A Amiri-Yekta A Zomorodipour H Gourabi M Shahriari khalaj MH Sanati N Fatemi S Abolghasemi S Bahrami

a:4:{s:10:"Background";s:897:"Human luteinizing hormone (hLH) belongs to glycoprotein hormones which is composed of two non-covalently linked subunit, α and β. The α-subunit is similar in all glycoprotein hormones, whereas the β-subunit is conferring the hormonal specificity. This hormone has important roles in the growth and maturity of sexual organs and secondary sexual characteristics and st...

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function paginate(evt) { url=/search_year_filter/ var term=document.getElementById("search_meta_data").dataset.term pg=parseInt(evt.target.text) var data={ "year":filter_year, "term":term, "pgn":pg } filtered_res=post_and_fetch(data,url) window.scrollTo(0,0); } function update_search_meta(search_meta) { meta_place=document.getElementById("search_meta_data") term=search_meta.term active_pgn=search_meta.pgn num_res=search_meta.num_res num_pages=search_meta.num_pages year=search_meta.year meta_place.dataset.term=term meta_place.dataset.page=active_pgn meta_place.dataset.num_res=num_res meta_place.dataset.num_pages=num_pages meta_place.dataset.year=year document.getElementById("num_result_place").innerHTML=num_res if (year !== "unfilter"){ document.getElementById("year_filter_label").style="display:inline;" document.getElementById("year_filter_place").innerHTML=year }else { document.getElementById("year_filter_label").style="display:none;" document.getElementById("year_filter_place").innerHTML="" } } function update_pagination() { search_meta_place=document.getElementById('search_meta_data') num_pages=search_meta_place.dataset.num_pages; active_pgn=parseInt(search_meta_place.dataset.page); document.getElementById("pgn-ul").innerHTML=""; pgn_html=""; for (i = 1; i <= num_pages; i++){ if (i===active_pgn){ actv="active" }else {actv=""} pgn_li="
  • " +i+ "
  • "; pgn_html+=pgn_li; } document.getElementById("pgn-ul").innerHTML=pgn_html var pgn_links = document.querySelectorAll('.mypgn'); pgn_links.forEach(function(pgn_link) { pgn_link.addEventListener('click', paginate) }) } function post_and_fetch(data,url) { showLoading() xhr = new XMLHttpRequest(); xhr.open('POST', url, true); xhr.setRequestHeader('Content-Type', 'application/json; charset=UTF-8'); xhr.onreadystatechange = function() { if (xhr.readyState === 4 && xhr.status === 200) { var resp = xhr.responseText; resp_json=JSON.parse(resp) resp_place = document.getElementById("search_result_div") resp_place.innerHTML = resp_json['results'] search_meta = resp_json['meta'] update_search_meta(search_meta) update_pagination() hideLoading() } }; xhr.send(JSON.stringify(data)); } function unfilter() { url=/search_year_filter/ var term=document.getElementById("search_meta_data").dataset.term var data={ "year":"unfilter", "term":term, "pgn":1 } filtered_res=post_and_fetch(data,url) } function deactivate_all_bars(){ var yrchart = document.querySelectorAll('.ct-bar'); yrchart.forEach(function(bar) { bar.dataset.active = false bar.style = "stroke:#71a3c5;" }) } year_chart.on("created", function() { var yrchart = document.querySelectorAll('.ct-bar'); yrchart.forEach(function(check) { check.addEventListener('click', checkIndex); }) }); function checkIndex(event) { var yrchart = document.querySelectorAll('.ct-bar'); var year_bar = event.target if (year_bar.dataset.active == "true") { unfilter_res = unfilter() year_bar.dataset.active = false year_bar.style = "stroke:#1d2b3699;" } else { deactivate_all_bars() year_bar.dataset.active = true year_bar.style = "stroke:#e56f6f;" filter_year = chart_data['labels'][Array.from(yrchart).indexOf(year_bar)] url=/search_year_filter/ var term=document.getElementById("search_meta_data").dataset.term var data={ "year":filter_year, "term":term, "pgn":1 } filtered_res=post_and_fetch(data,url) } } function showLoading() { document.getElementById("loading").style.display = "block"; setTimeout(hideLoading, 10000); // 10 seconds } function hideLoading() { document.getElementById("loading").style.display = "none"; } -->