A class of RNA in HeLa cytoplasm with a sedimentation value of 22S is described. This RNA is a true cytoplasmic component and is not an artifact of cell rupture or of the method of RNA preparation. The 22S RNA sediments in a sucrose gradient with those ribosomal structures containing 28S ribosomal RNA (60S and 74S particles and polyribosomes). It has a base composition and methyl content simila...

The R1Bm element, found in the silkworm Bombyx mori, is a member of a group of widely distributed retrotransposons that lack long terminal repeats. Some of these elements are highly sequence-specific and others, like the human L1 sequence, are less so. The majority of R1Bm elements are associated with ribosomal DNA (rDNA). R1Bm inserts into 28S rDNA at a specific sequence; after insertion it is...

conclusions it was found that the isolated bacteria produced biosurfactants that had a great potential for different industries. results the 3 isolates from cheese, yoghurt and silage were effective against isolated e.coli and could produce biosurfactants. phylogenic relationships of the 3 potential candidates were determined comparing the 16sr dna gene sequences, they were found to be as 3 iso...

Zinc is essential for growth and reproduction of animals and i t has been suggested that the most critical requirement for Zn is for alteration in the genetic expression of cells (Chesters, 1978). However, in EDTA-treated lymphocyte cultures, lack of Zn delayed maturation of 32s ribosomal RNA precursor to 28s ribosomal RNA and reduced survival of the 28s RNA (Chesters, 1975). This alteration in...

Total RNA isolated by Laser Capture Microdissection (LCM) from embryonic kidney cells was analyzed for integrity and relative concentration using the Agilent 2100 bioanalyzer and the RNA 6000 Pico assay. An unexpected peak between the 18S and 28S ribosomal RNA peaks was observed and unambiguously proven, by DNase digestion and genomic DNA spike-in experiments, to be caused by genomic DNA contam...

This study documents a new deep-sea chiton from the Haima cold seeps. Thermochiton xui . nov. is third species of genus and first occurrence this in South China Sea. identified by its morphological characteristics molecular sequence reported for time. The placement determined phylogenetic tree Ischnochitonidae Maximum Likelihood (ML) Bayesian inference (BI) methods, based on sequences mitochond...

A new species of Bembidion (Trichoplataphus Netolitzky) from the Ozark Plateau of Missouri and Arkansas is described (Bembidion ozarkense Maddison and Hildebrandt). It is distinguishable from the closely related species, Bembidion rolandi Fall, by characteristics of the male genitalia, and sequences of the genes cytochrome oxidase I and 28S ribosomal DNA. A brief review of the North American sp...

Phylogenetic analysis of 1656 aligned sites in the 28S ribosomal RNA gene of frogs supports some of the recently recognized higher groups of anurans but provides counter-support for others. The 28S rDNA data support the monophyly of the recently recognized Pipanura (mesobatrachians plus neobatrachians), which in turn indicates paraphyly of archaeobatrachians. Mesobatrachians (pelobatoids plus p...

The utility of various regions of the ribosomal repeat unit for phylogenetic analysis was examined in 16 species representing four families, nine genera, and two orders of the subclass Copepoda (Crustacea). Fragments approximately 2000 bp in length containing the ribosomal DNA (rDNA) 18S and 28S gene fragments, the 5.8S gene, and the internal transcribed spacer regions I and II (ITS1 and ITS2) ...