Membranes rich in acetylcholine receptor were isolated from Torpedo californica by a modification of the procedure of Sobel et al. (Sobel, A., Weber, M., and Changeux, J.-P. (1977) Eur. J. Biochem. 80, 215-224). The receptor was extracted with 2% potassium cholate in the presence of 2.5% soybean phospholipids. After reconstitution by the cholate dialysis procedure, the vesicles exhibited a rapi...

Purified acetylcholine receptor and total lipids, both extracted from Torpedo californica electroplax, were utilized to reconstitute chemically excitable membrane vesicles. Reconstitution was achieved by dialysis of the extraction detergent, octyl beta-D-glucoside from protein/lipid incubation mixtures. The reconstituted preparations could be fractionated by sucrose density gradient centrifuga...

The present study concerns the medical applications and production of 52Fe via reaction 55Mn(p,4n)52Fe together with reference 27Al(p,x)22Na employed in cross section measurements. Experimental excitation functions from threshold up to 200 MeV are compared predictions TALYS 1.95 code semi-empirical formulas SPACS Silberberg & Tsao. We obtained two parameter sets for reactions which give a g...

Sodium (Na) exclusion is vital for plants to cope with salt stress, and the plasma membrane Na+/H+ antiporter, SALT OVERLY SENSITIVE 1 (SOS1), has been known play a central role in this process although function of molecule not completely understood. In study, we performed 22Na+ radiotracer experiments investigate SOS1 mature root zone. Arabidopsis thaliana seedlings were placed on gel medium, ...

The positron annihilation lifetimes were measured using a 48V source in noncentrosymmetric cubic single crystals of CoSi, FeSi and MnSi. following determined from the time spectra: 168(1) ps for 114(1) 111(1) For single-crystal lifetime was also with 22Na source. obtained different sources are consistent. differences MnSi FeSi, on one hand, Weyl semimetal other possibly caused by formation + el...