Based on available toxicity data, malondialdehyde (MDA; O=CHCH2CH=O) has been designated as a potential human carcinogen. A handful of studies suggest that MDA forms in the gas and aerosol phase troposphere, potentially contributing to inhalation toxicity, yet it never quantified ambient air. The thiobarbituric acid (TBA) assay for (MDA) used marker reactive oxygen species (ROS), oxidative stre...

Low density lipoprotein (LDL) isolated from human plasma anticoagulated with EDTA (EDTA/LDL) was 4-fold more resistant to oxidation by reagent H2O2, as assayed by the thiobarbituric acid (TBA) assay, than LDL prepared from plasma anticoagulated with citrate (CDP/LDL). The LDLs required 1-3 mM H2O2 for maximal oxidation by this assay, and ED50S were 1.7 X 10(-3) M for EDTA/LDL and 4.5 X 10(-4) M...

Cerebrospinal fluid lipoperoxides, measured as the malondialdehyde-thiobarbituric acid (MDA-TBA) adduct, were quantified by adapting the plasma liquid-chromatographic method of Wong et al. (Clin Chem 1987;33:214-20) to cerebrospinal fluid. Reference values for spinal fluid specimens from 91 adults, ages 17 to 95 y, and 37 children, ages 8 d to 8 y, were determined. Their concentrations were not...

Abbreviations: B2M, β-2-microglobulin; BACT, β-actin; BHT: butylated hydroxytoluene; 10 cDNA, complementary DNA; CL, cardiolipin; COX, cytochrome c oxidase complex; DHA, 11 docosahexaenoic acid; ETC, electron transport chain; FA, fatty acid; FAME, fatty acid methyl 12 esters; HP-TLC, high performance thin layer chromatography; LA, linoleic acid; LC-PUFA, 13 long chain polyunsaturated fatty acid...

Silver carp fillets were treated with liposomal encapsulated and unencapsulated pomegranate peel extract at 0.5 and 1% w/w to evaluate the effect of the extracts on the shelf life of the fillets during 15 days of refrigerated storage. Quality changes of the samples were investigated in terms of total volatile basic nitrogen (TVB-N), peroxide value (PV), thiobarbituric acid (TBA) and microbial c...

The study was conducted to find out the effect of adding different levels sodium alginate on sensory, physicochemical, biochemical and microbiological properties fresh preserved chicken sausage. Chicken sausage samples were divided into three treatment groups: T1 (Control group), T2 (2% alginate) T3 (4% alginate). sensory qualities like color, juiciness, tenderness overall acceptability increas...

Electrochemical oxidation of iodide has been studied in the presence of 2-thioborbituric acid using cyclic voltammetry and controlled-potential coulometry. The results indicate that, the resulting iodine takes part in halogenations reaction with 2-thiobarbituric acid. In addition, the present data are indicative of the suitability of iodide as a quasi mediator for determination of 2-thiobar...

Among the cellular molecules, lipids that contain unsaturated fatty acids with more than one double bond are particularly susceptible to action of free radicals. The resulting reaction, known as lipid peroxidation, disrupts biological membranes and is thereby highly deleterious to their structure and function. Lipid peroxidation is being studied extensively in relation to disease, modulation by...

The effect of aqueous solutions of ascorbic acid (AA) on the rancidity development in Cobia (Rachycentron canadum) fillets during frozen storage was studied. Cobia fillets were treated with ascorbic acid (AA 0.25% and AA 0.5%) then stored at -18°C up to 6 months. Rancidity development was measured by several biochemical indices including free fatty acids (FFA), peroxide value (PV), and thiobarb...

The effect of aqueous solutions of ascorbic acid (AA) on the rancidity development in Cobia (Rachycentron canadum) fillets during frozen storage was studied. Cobia fillets were treated with ascorbic acid (AA 0.25% and AA 0.5%) then stored at -18°C up to 6 months. Rancidity development was measured by several biochemical indices including free fatty acids (FFA), peroxide value (PV), an...