The chemical nature of the important new chromogen ABTS [2,2'-azino-di-(3-ethyl-benzthiazoline-6-sulphonic acid)] is described together with an account of the redox and spectroscopic properties of the system ABTS--H2O2--peroxidase. Keq. is calculated and a study of the steady-state kinetics over a whole range of substrate concentrations is reported. By using novel methods of kinetic analysis, a...

Soluble flaxseed gum (SFG) was extracted from flax (Linum usitatissimum) hulls using hot water, and its functional groups and antioxidant properties were investigated using infrared spectroscopy and different antioxidant assays (2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid (ABTS), reducing power capacity, and β-carotene bleaching inhibition assay)...

Jujube is a crop very resistant to drought and salinity, making it an interesting growing alternative in southeastern Spain. The characteristics of five different cultivars the jujube fruit have been evaluated classified into four maturation stages according color peel, ranging from green its most immature stage, white, yellow, red last, more mature stage. This due part amount carotenoids chlor...

To assess the impacts of Cu and Zn on fungi, we tested the growth of 18 taxonomically different saprotrophic (basidiomycete, ascomycete and zygomycete) fungi and their production of extracellular oxidative enzymes on Cu(0, 100, 200, 400 mg kg–1) or Zn(0, 100, 200, 400 mg kg–1) containing ABTS (2,2 ́-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) agar plates. Coniothyrium sp. was the most toler...

In order to understand antidiabetic potential and toxicity, this study aimed evaluate the acute toxicity activity of ethanolic extract ethyl acetate fraction obtained from Coutoubea spicata “nicolau” shoots. Chemical constituents were investigated. alloxan-induced diabetic rats, tested at dose 100 mg/kg, p.o. Body weight gain, glucose, lipid profile oxidative stress markers in serum tissues det...

Submit Manuscript | http://medcraveonline.com Abbreviations: CPITN, communiy periodontal index treatment needs; PSR, periodontal screening & recording; hBD 1, human beta defensin 1; hBD2, human beta defensin 2; LPS, lipopolysaccharides; TNF-α, tumor necrosis factor-alpha; IL-1β, interleukin-1 beta; ELISA, enzyme-linked immunosorbent assay; PBS, phosphate buffered saline; ABTS, 2’-azino-bis-3-et...

Coffee is one of the most popular beverages in the world, prepared and consumed in many different ways. Taste, aroma and composition of the coffee brew vary depending on the preparation method. Therefore, this study investigates the effect of different brewing methods on the polyphenol and methylxanthine composition and antioxidant capacity of thirteen different coffee brews. The content of tot...

Background The search for new natural or synthetic products with antioxidant activity is commonly based on methods that involve reduction of either 2,2-diphenyl-1-picrylhydrazyl (DPPH) or 2-2-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS). However, the reported values of the effective concentrations are highly variable, even in controls. Herein, we optimize and validate both meth-ods...

The supercritical fluid extraction (SFE) of Lepidium apetalum seed oil and its anti-oxidant activity were studied. The SFE process was optimized using response surface methodology (RSM) with a central composite design (CCD). Independent variables, namely operating pressure, temperature, time and flow rate were evaluated. The maximum extraction of Lepidium apetalum seed oil by SFE-CO₂ (about 36....

Picrorhiza kurroa is a well-known herb in Ayurvedic medicine. Although it shows antioxidant, antiinflammatory and immunomodulatory activities, it is most valued for its hepatoprotective effect. The rhizomes are widely used against indigestion problems since ancient times due to improper digestive secretions. Aim of this study was to explore antioxidant study of P. kurroa leaves for a new source...