In the present work, we analyzed whether polybrominated diphenyl ethers (PBDEs) (47, 99, 100 and 209) interfere with the effect of 17β-estradiol on the proliferation and apoptosis of the MCF-7 cell line. MCF-7 cells were cultured in DMEM without phenol red; upplemented with 5% charcoal-treated fetal bovine serum for 3 days with 10 nM 17β-estradiol; with 0.1 μM, 0.5 μM or 1 μM of the tested PBDE...

Bladder cancer belongs to one of the most common cancers and is a leading cause of deaths in our society. Urothelial carcinoma of the bladder (UCB) is the main type of this cancer, and the estrogen receptors in UCB remain to be studied. Our experiment aimed to investigate the possible biological effect of 17β-estradiol on human bladder-derived T24 carcinoma cells and to indicate its related mec...

BACKGROUND 17β-estradiol can suppress acute lung injury (ALI) and regulate alveolar epithelial sodium channel (ENaC). However the relationship between these two functions remains unclear. This study is conducted to assess the role of ENaC and the PI3K/Akt/SGK1 signaling pathway in 17β-estradiol therapy in attenuating LPS-induced ALI. METHODS ALI was induced in C57BL/J male mice by intratrache...

A delayed oligodendrocyte cell death after spinal cord injury (SCI) contributes to chronic demyelination of spared axons, leading to a permanent neurological deficit. Therefore, therapeutic approaches to prevent oligodendrocyte cell death after SCI should be considered. Estrogens are well known to have a broad neuroprotective effect, but the protective effect of estrogens on oligodendrocytes af...

Emerging evidence suggests that renal endothelial function may be altered in ischemia-reperfusion injury. Acute kidney injury is sexually dimorphic, and estrogen protects renal tubular function after experimental ischemic injury. This study tested the hypothesis that during ischemia-reperfusion, estrogen alters glomerular endothelial function to prevent hyperpermeability. Glomerular endothelial...

Estrogens regulate dendritic spine density, but the mechanism and significance of this effect for brain networks remain unknown. We used repetitive imaging over several days to investigate how 17β-estradiol affected the turnover and long-term behavior of dendritic spines in CA1 cells of hippocampal slice cultures. We find that 17β-estradiol and serum in the culture medium tightly regulated spin...

The presence and location of specific binding sites for progesterone and 17β-estradiol in cells of wheat were estimated using radioligand binding assay. Membrane and cytosolic fractions of non-vernalized and vernalized plants were tested using tritium-labelled ligands. Specific binding of [3H]progesterone and [3H]17β-estradiol occurs in wheat cells. The binding sites are located in membranes an...

introduction: 17&beta-estradiol; modulates nociception by binding to the estrogenic receptors and also by allosteric interaction with other membrane-bound receptors like glutamate and gabaa receptors. in addition to its autonomic functions, paragigantocellularis lateralis (lpgi) is involved in the pain modulation, too. the aim of the current study was to investigate the involvement of the membr...

The objective of this study was to characterize the circulating concentrations of estradiol-17β (E-17β) after treatment with different types or doses of estrogens in the absence (Experiment 1) or presence (Experiment 2) of a dominant follicle in lactating cows. In Experiment 1, cows (n = 12) had all follicles > 5 mm removed by ultrasound-guided follicular aspiration every 12 h throughout the bl...

Background: The pathomechanisms of gestational trophoblastic disease have not yet been able to be extensively understood. It is associated with oxidative damage related to the production of reactive oxygen species (ROS). Estrogen, as well as all of its metabolites, can produce ROS. Therefore it is necessary to analyze how far the involvement of estrogen, in this case 17β estradiol, to the patho...