In prokaryotes, the ribosomal gene locus contains three rRNA species: 16S, 23S and 5S. The potential of sequencing 16S rDNA of bacteria and archaea for establishing phylogenetic relationships and for use in molecular diagnostics is well-documented. Currently, over 3000 16S rRNA sequences are available in the databases. However, the potential of the 23S rRNA gene as a diagnostic tool has not bee...

BACKGROUND Staphylococcus aureus is a common and significant pathogen in cystic fibrosis. We sought to determine if quantitative PCR (qPCR) and 16S rRNA gene sequencing could provide a rapid, culture-independent approach to the identification of S. aureus airway infections. METHODS We examined the sensitivity and specificity of two qPCR assays, targeting the femA and 16S rRNA gene, using cult...

The development and continuous improvement of high-throughput sequencing platforms have stimulated interest in the study of complex microbial communities. Currently, the most popular sequencing approach to study microbial community composition and dynamics is targeted 16S rRNA gene metabarcoding. To prepare samples for sequencing, there are a variety of processing steps, each with the potential...

Routine blood analysis indicated the presence of Mycoplasma-like bodies in a guigna (Leopardus guigna). Evidence infection with Candidatus Mycoplasma haemominutum was found samples using PCR and DNA sequencing 16S rRNA gene Mycoplasma. spp. are documented cats but their role transmission to populations requires investigation.

The present study deals with isolation, identification and analysis of bacteria from tannery effluent through 16S rRNA based molecular technique. Bacterial strain was isolated and characterized using various biochemical tests and confirmed through molecular approach. Bacterial 16S rRNA gene was amplified using suitable primers. The amplified 16S rRNA gene sequence was compared with the sequence...

Motivation: The characterization of phylogenetic and functional diversity is a key element in the analysis of microbial communities. Amplicon-based sequencing of marker genes, such as 16S rRNA, is a powerful tool for assessing and comparing the structure of microbial communities at a high phylogenetic resolution. Because 16S rRNA sequencing is more cost-effective than whole metagenome shotgun s...

16S rRNA molecules in a microbial strain can differ due to nucleotide variation between their genes. This is a typical trait of fast-growing bacteria to cope with different niches. We investigated characteristics of 16S rRNA genes in Vibrio splendidus strain PB1-10, from the normal flora of Atlantic halibut. Sequencing of 16S rRNA gene clones detected 35 variable positions in a total of 13 diff...