Wild populations of Medicago ciliaris and Medicago polymorpha were subjected to four salt treatments 0, 50, 100 and 150 mM NaCl, plant growth and proline concentration in leaves were assessed. The analyzed data revealed significant variability in salt response within and between the two species, depending on the salinity level. It was found that high NaCl concentrations affected all the growth ...

salinity is a major limiting factor for plant growth and development. to evaluate the impact of p5cs gene expression under in vitro salt stress condition, transgenic tobacco (nicotiana plumbaginifolia) carrying p5cs gene and non-transgenic plants were treated with 0, 100, 150, 200 or 250 mm nacl for 28 days. proline content, lipid peroxidation and the activity of some antioxidant enzymes after ...

Pyruvate kinase, lactate dehydrogenase, and phosphoenol pyruvate were purchased from Sigma. Restriction endonucleases, shrimp alkaline phosphatase (SAP), and T4 polynucleotide kinase were from New England Biolabs. Covalently closed circular χ +-3F3H dsDNA (a pBR322 derivative, containing tandem χ-sequences (Anderson et al., 1999)) was purified using a QIAGEN " Maxi kit ". M13mp7 replicative for...

the influence of salinity on the different polyamine fractions (free, conjugated, and bound) was compared in a natrophilic halophyte (beta vulgaris l. cv. ic) and a salt sensitive glycophyte (nicotiana rustica l. cv. basmas). low-level salinity (25 mm nacl) and high salinity (150 and 50 mm nacl for sugar beet and tobacco, respectively) were supplied in hydroponics. under low salinity shoot dry ...

In this study, the effects of pre-germination at different doses acetyl salicylic acid (ASA) solution on germination and emergence performance “Nantes” carrot cultivar (Daucus corata L.) seeds under salt stress were investigated in laboratory conditions. Seeds pre-germinated a climate cabinet for 24 hours 0, 50, 100 150 mg L-1 ASA solutions hydropiriming 15oC. After process, they divided into t...

Ca2+-free calmodulin was obtained by exhaustively dialyzing 2 mg protein (c = 4 mg/mL) against 1000 volumes of 2 mM EGTA, 50 mM Tris HCl and 150 mM NaCl (pH = 7.4) at 4 °C in a 0.5 mL Pierce Slide-A-LyzerTM Dialysis Cassette (3.5 K MWCO) for 2 days. As the carboxylic groups of EGTA exhibit strong IR bands in the region 1570-1630 cm-1 that overlap with the amide I' band of proteins (1600-1700 cm...

The contribution of the organum vasculosum laminae terminalis (OVLT) in mediating central hyperosmolality-induced increases of sympathetic nerve activity (SNA) and arterial blood pressure (ABP) was assessed in anesthetized rats. Solutions of graded NaCl concentration (150, 375, and 750 mM) were injected (150 mul) into the forebrain vascular supply via an internal carotid artery (ICA). Time-cont...

Engineering of salinity tolerance in agronomically important crop plants is required to increase their productivity by enabling them to grow in saline soils, which are otherwise left uncultivated. Since an increase in the enzymes of glyoxalase system has been shown to impart salinity tolerance in the model plant tobacco, we used the glyoxalase II gene for engineering salinity tolerance in an im...

In this study, it was aimed to investigate the effects of salinity, which is an important environmental problem, in cultivation ornamental plants (such as zinnia) and irrigation with high salt water, especially on antioxidant defense mechanism. For purpose, two Zinnia species were irrigated by different concentrations saline water (50, 100, 150, 200 mM NaCl); salinity superoxide dismutase (SOD)...