Proteins have short half-life because of enzymatic cleavage. Here, a new protein nanocarrier made of graphene oxide (GO) + Chitosan (CS) is proposed to successfully prevent proteolysis in protein and simultaneously retain its activity. Bovine serum albumin (BSA) and collagenase were loaded on GO and GO-CS to explore the stability and activity of proteins. SEM, AFM, TEM, DSC, UV-Vis, FT-IR, RBS,...

for induction of ruminal acidosis, 10 clinically healthy three years old non pregnant female sheep were selected. prior to the infusion of sucrose (0 hour), rumen and blood samples were obtained in order to determine baseline rumen and blood ph, respectively. electrocardiogram (ecg) was also recorded. acute ruminal acidosis was induced experimentally with sucrose at a dose of 18g kg-1 body weig...

It is now possible to store turkey semen for 4 to 24 h without appreciable loss in fertility and hatchability. Optimum conditions for 24 or 48 h storage have not been fully determined. Development of new storage media, allowing the insemination of very low numbers of spermatozoa, may provide interesting possibilities regarding the use of superior sires. This study was conducted to evaluate effe...

purpose: to compare post-operative pain with cooling photorefractive keratectomy (prk) versus conventional prk. methods: a prospective clinical trial was performed on 94 eyes from 47 patients scheduled for prk. patients were randomly assigned to either cooling or conventional prk. pain was evaluated 6, 12, 24 and 48 hours post-operation using a visual analogue scale (vas). corneal haze, conjunc...

The effect of intraperitoneal administration of a single dose (1μg/g) of thyroxine (T4) after 24, 48 and 98 hours on plasma total proteins, albumin fraction, uric acid, glucose, triglycerides and cholesterol has been investigated in broiler (Hubbard) chicks of 15, 25 and 35 days of age. Thyroxine treatment resulted in a significant decrease of plasma proteins after 96 hours of the experiment in...

the aim of this study was to determine the quality of cumulus-oocyte-complexes (cocs) and the effects of bovine serum albumin (bsa) supplementation on in vitro maturation and fertilization rate of buffalo oocytes. cocs were collected from slaughterhouse buffalo ovaries by aspiration method. only normal grades cocs were matured for 48 hours in tcm-199 media. two groups were created: one for the ...