The methylotrophic yeast Pichia pastoris is now an established expression system for the production of recombinant protein for nuclear magnetic resonance (NMR) studies. It is capable of expressing high levels of heterologous proteins and possesses the ability to perform many of the posttranslational modifications of higher eukaryotes. Here, we describe efficient methods for the production of un...

High-resolution 13C MAS NMR spectroscopy was used to profile a range of primary and secondary metabolites in vivo in intact whole seeds of eight different conifer species native to North America, including six of the Pinaceae family and two of the Cupressaceae family. In vivo 13C NMR provided information on the total seed oil content and fatty acid composition of the major storage lipids in a n...

Sir: AR-5 (Mycinamicins) antibiotics, 16-membered ring macrolides, are produced by Micromonospora sp. These novel macrolides are potent antibiotics with unusual activity. Structures of these compounds were independently assigned by us and a group of Japanese workers.',') The assignments of the signals in the 13C NMR spectra have been previously reported". In analogy with the origin of the carbo...

In order to facilitate the study of motions of the carbohydrate moiety of glycoproteins, a method of enzymatically attaching uniformly 13C-enriched galactopyranose ([U-13C]galactose) onto the nonreducing end of the carbohydrate chain has been developed. Specifically, [U-13C]glucose (20% 13C) was converted through a sequence of four enzymatic steps to [U-13C]UDP-galactose which was then attached...

Advanced solid-state NMR was employed to investigate differences in chemical structure and heterogeneity between milled wood lignin (MWL) and residual enzyme lignin (REL). Wiley and conventional milled woods were also studied. The advanced NMR techniques included 13C quantitative direct polarization, various spectral-editing techniques, and two-dimensional 1H-13C heteronuclear correlation NMR w...

Broussonetines are glycosidase-inhibitory alkaloids obtained from Broussonetia kazinoki. Feeding experiments using [1-13C]glucose and 13C-NMR spectroscopic studies showed that broussonetines are biosynthesized through routes similar to those of sphingosine and phytosphingosine.

Eight known compounds were isolated from the shrub Tephrosia toxicaria. Among them, 6,7-dimethoxy-chromone (1), was described by the first time from this genus, villosinol (2), which had been previously reported without its 13C-NMR data and sumatrol (3), which has its 13C-NMR data corrected. The antibacterial activity of Tephrosia toxicaria extract and obovatin (6), deguelin (7), 12a-hydroxyα-t...

The electrochemical oxidation of methyl catechol in the presence of 2-thiouracil (as a nucleophile) has beenperformed in aqueous solutions using cyclic voltammetry and controlled-potential coulometric techniques.The electrochemical behavior of methyl catechol in the presence of 2-thiouracil was investigated. Theelectrochemical synthesis of this compound has been successfully performed in an und...