Clustered regularly interspaced short palindromic repeats-associated protein 9 (CRISPR-Cas9) is a revolutionary technology that enables efficient genomic modification in many organisms. Currently, the wide use of Streptococcus pyogenes Cas9 (SpCas9) primarily recognizes sites harbouring a canonical NGG protospacer adjacent motif (PAM). The newly developed VQR (D1135V/R1335Q/T1337R) variant of C...

BRAF-V600E (1799T > A) is one of the most frequently reported driver mutations in multiple types of cancers, and patients with such mutations could benefit from selectively inactivating the mutant allele. Near this mutation site, there are two TTTN and one NGG protospacer-adjacent motifs (PAMs) for Cpf1 and Cas9 CRISPR nucleases, respectively. The 1799T > A substitution also leads to the occurr...

Genome editing technologies based on RNAguided nucleases (RGNs) derived from prokaryotic type II CRISPR-Cas9 adaptive immune systems, such as that from Streptococcus pyogenes and, more recently, Staphylococcus aureus, are becoming increasingly pervasive in both basic and applied research. RGNs are ribonucleoprotein complexes whose sequence-specific guide RNA (gRNA) moieties address a Cas9 nucle...

This paper presents theory for Normalized Random Measures (NRMs), Normalized Generalized Gammas (NGGs), a particular kind of NRM, and Dependent Hierarchical NRMs which allow networks of dependent NRMs to be analysed. These have been used, for instance, for time-dependent topic modelling. In this paper, we first introduce some mathematical background of completely random measures (CRMs) and thei...

We present the results of a long-term HiRes spectroscopic campaign on the O-type stars in NGC 6231. We revise the spectral classification and multiplicity of these objects and we constrain fundamental properties of the O-star population. Almost three quarters of the O-type stars in the cluster are members of a binary system. The minimum binary fraction is 0.63, with half the O-type binaries hav...

Catechol is used in many industries. It can be removed from wastewater by various methods but biological processes are the most superior and commonly used technology. The SCR is a modified form of SBR used to degrade catechol. The objective of this study was to investigate the performance of SCR for biodegradation and mineralization of catechol under various inlet concentrations (630-1500 mg/L)...

Structure-based topological descriptors of chemical networks enable us the prediction physico-chemical properties and bioactivities compounds through QSAR/QSPR methods. Topological indices are numerical values to represent a graph which characterises graph. One latest distance-based index is Mostar index. In this paper, we study index, Szeged PI <math xmlns="http://www.w3.org/1998/Math/MathML" ...

The seasonal variations in the occurrence and distribution of pharmaceuticals were evaluated in seawater and sediment of Mar Menor lagoon from spring 2010 to winter 2011. A total of 20 pharmaceuticals in seawater and 14 in sediments were found at concentrations from low ngL(-)(1) up to 168ngL(-)(1) (azithromycin) in seawater and from low ngg(-1) up to 50.3ngg(-1) (xylazine) in sediments. Azithr...